| 紫外分光光度法测定当归补血汤中的总苷含量 |
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| 引用本文： | 严安定,高建,王培培,夏泉,许杜娟.紫外分光光度法测定当归补血汤中的总苷含量[J].安徽医学,2011,32(5):636-638. |
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| 作者姓名： | 严安定 高建 王培培 夏泉 许杜娟 |
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| 作者单位： | 安徽医科大学第一附属医院药剂科,国家中医药管理局中药化学三级实验室(TCM-2009202),合肥,230022;230022,合肥,安徽医科大学第一附属医院药剂科,国家中医药管理局中药化学三级实验室(TCM-2009202);230032,合肥,安徽医科大学药学院 |
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| 基金项目： | 国家自然科学基金资助项目，安徽省高校省级教学质量与教学改革工程项目 |
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| 摘 要： | 目的 建立用紫外分光光度法测定当归补血汤中总苷含量的方法.方法 采用紫外分光光度法,以黄芪甲苷为对照品,在波长540 nm处测定当归补血总苷含量.结果 黄芪甲苷的线性范围为4.167-41.667 μg*ml-1(r2= 0.9986),平均回收率为99.3%,RSD=0.74% (n=5).结论 本方法准确、简便、快...
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| 关 键 词： | 紫外分光光度法 当归补血总苷 黄芪甲苷 含量测定 |
Determination of the total glucosides of danggui buxue tang by UV spectrophotometry |
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| Yan ,ing,Gao Jian,Wang Peipei,et al The First Affiliated Hospital of Anhui Medical University,the Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine,Hefei ,China.Determination of the total glucosides of danggui buxue tang by UV spectrophotometry[J].Anhui Medical Journal,2011,32(5):636-638. |
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| Authors: | Yan ing Gao Jian Wang Peipei The First Affiliated Hospital of Anhui Medical University the Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine Hefei China |
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| Institution: | Yan Anding,Gao Jian,Wang Peipei,et al The First Affiliated Hospital of Anhui Medical University,the Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine,Hefei 230022,China |
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| Abstract: | Objective To establish a method for the determination of the total glucosides of Danggui Buxue tang by UV spectrophotometry.Methods Based on the reference substance of astragloside IV,the determination was performed by UV spectrophotometry at the wavelength of 540 nm.Results The calibration culve of the absorbence was linear with the concentration of astragloside IV in the range of 4.167-41.667μg·ml-1(r2=0.9986).The average recovery rate of astragloside IV was 99.3% with the RSD of 0.74%(n=5).Conclusion The method is accurate,simple,fast,feasible and can be applied to the determination of total glucosides of Danggui Buxue tang. |
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| Keywords: | UV Spectrophotometry Total Glucosides of Danggui Buxue Tang Astragloside IV Determination |
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