| 蒙药“通拉嘎-5”对脂肪细胞葡萄糖摄取的影响及其机制研究 |
| |
| 引用本文： | 陈刘,王慧,姜友昭,包纳日斯,陈兵.蒙药“通拉嘎-5”对脂肪细胞葡萄糖摄取的影响及其机制研究[J].第三军医大学学报,2011,33(7):686-689. |
| |
| 作者姓名： | 陈刘 王慧 姜友昭 包纳日斯 陈兵 |
| |
| 作者单位： | 陈刘,王慧,姜友昭,陈兵,Chen Liu,Wang Hui,Jiang Youzhao,Chen Bing(第三军医大学西南医院内分泌科,重庆,400038);包纳日斯,Bao Narisi(成都中医药大学民族医药学院,成都,610075) |
| |
| 基金项目： | 国家自然科学基金面上项目 |
| |
| 摘 要： | 目的探讨"通拉嘎-5"对3T3-L1脂肪细胞葡萄糖摄取的影响及其作用机制。方法将3T3-L1前脂肪细胞诱导分化为脂肪细胞后,"通拉嘎-5"作用于3T3-L1脂肪细胞24 h,用3H标记的2-脱氧-葡萄糖(3H]2-DG)示踪检测胰岛素介导的3T3-L1脂肪细胞摄取葡萄糖的情况;"通拉嘎-5"作用3T3-L1脂肪细胞48 h后,用Real-time PCR检测PPARγ和LXRα的基因表达,用Western blot检测PPARγ和LXRα的蛋白表达。结果 "通拉嘎-5"能促进胰岛素介导的3T3-L1脂肪细胞的葡萄糖摄取(比阴性对照组高1.915倍,P<0.05);能明显上调3T3-L1脂肪细胞和LXRα基因(比阴性对照组高2.895倍,P<0.01)和蛋白(比阴性对照组高1.977倍,P<0.01)表达;而对PPARγ基因和蛋白表达无明显的影响。结论 "通拉嘎-5"可能通过提高3T3-L1脂肪细胞LXRα的基因和蛋白表达,促进胰岛素介导的葡萄糖摄取功能,改善胰岛素抵抗,可能为"通拉嘎-5"治疗非酒精性脂肪肝(NAFLD)的作用机制之一。
|
| 关 键 词： | 通拉嘎-5 3T3-L1 葡萄糖摄取 PPARγ LXRα |
Mongolian drug Tonglaga-5 improves glucose uptaking in 3T3-L1 adipocytes |
| |
| Chen Liu,Wang Hui,Jiang Youzhao,Bao Narisi,Chen Bing.Mongolian drug Tonglaga-5 improves glucose uptaking in 3T3-L1 adipocytes[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(7):686-689. |
| |
| Authors: | Chen Liu Wang Hui Jiang Youzhao Bao Narisi Chen Bing |
| |
| Institution: | Chen Liu1,Wang Hui1,Jiang Youzhao1,Bao Narisi2,Chen Bing1(1Department of Endocrinology,Southwest Hospital,Third Military Medical University,Chongqing,400038,2College of Minority Traditional Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan Province,610075,China) |
| |
| Abstract: | Objective To investigate the effect and mechanism of Mongolian drug "Tonglaga-5" on the glucose uptaking in 3T3-L1 adipocytes.Methods 3T3-L1 adipocytes derived from 3T3-L1 preadipocytes differentiation were co-cultured with 27.27 mg/L "Tonglaga-5" for 24 h.Insulin mediated glucose uptaking in 3T3-L1 adipocytes was detected by 3H-labeled deoxyglucose.After 3T3-L1 adipocytes coculture with "Tonglaga-5" for 48 h,the mRNA expressions of PPARγ and LXRα were determined by Real-time PCR,and their protein expressions were determined by Western blotting.Results Insulin mediated glucose uptaking was increased by 1.915 times in 3T3-L1 adipocytes after Tonglaga-5 treatment when compared with normal control(P<0.05).While the expression of LXRα was increase by 2.895 times at mRNA level and by 1.977 times at protein level(P<0.01).But Tonglaga-5 treatment had no obvious effect on mRNA and protein expressions of PPARγ.Conclusion Tonglaga-5 may improve the insulin mediated glucose uptaking by up-regulating LXRα at both mRNA and protein levels in 3T3-L1 adipocytes,and then ameliorate insulin resistance. |
| |
| Keywords: | Tonglaga-5 3T3-L1 adipocytes glucose uptaking PPARγ LXRα |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
