| 杜氏利什曼原虫无鞭毛体蛋白基因原核表达重组质粒的构建 |
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| 引用本文： | 李金福,陈建平,田玉,马莹,胡孝素.杜氏利什曼原虫无鞭毛体蛋白基因原核表达重组质粒的构建[J].贵阳医学院学报,2011,36(2):111-114. |
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| 作者姓名： | 李金福 陈建平 田玉 马莹 胡孝素 |
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| 作者单位： | 1. 贵阳医学院寄生虫学教研室,贵州贵阳,550004
2. 四川大学华西基础医学与法医学院寄生虫学教研室,四川成都,610041 |
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| 基金项目： | 国家自然科学基金项目，贵州省科学技术基金 |
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| 摘 要： | 目的:构建杜氏利什曼原虫无鞭毛体蛋白(amastin)编码基因的原核表达重组质粒pET-amastin.方法:提取杜氏利什曼原虫基因组DNA进行PCR扩增,将扩增出的无鞭毛体蛋白基因片段导入质粒载体pET-32a(+),构建原核表达重组质粒pET-amastin.结果:扩增出大小约550 bp的无鞭毛体蛋白基因,重组质...
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| 关 键 词： | 利什曼原虫 杜氏 无鞭毛体蛋白 原核表达 基因克隆 |
Construction of Prokaryotic Expression Recombinant Plasmid of Amastin Gene of Leishmania donovani |
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| LI Jinfu,CHEN Jianping,TIAN Yu,MA Ying,HU Xiaosu.Construction of Prokaryotic Expression Recombinant Plasmid of Amastin Gene of Leishmania donovani[J].Journal of Guiyang Medical College,2011,36(2):111-114. |
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| Authors: | LI Jinfu CHEN Jianping TIAN Yu MA Ying HU Xiaosu |
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| Institution: | LI Jinfu1,CHEN Jianping2,TIAN Yu2,MA Ying2,HU Xiaosu2(1.Department of Parasitology,Guiyang Medical College,Guiyang 550004,Guizhou,China,2.Department of Parasitology,School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,Sichuan,China) |
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| Abstract: | Objective: To construct prokaryotic expression recombinant plasmid of amastin gene of Leishmania donovani.Methods: Amastin gene in nuclear DNA of L.donovani was isolated and amplified which was then cloned into prokaryotic expression vector pET-32a(+) to construct recombinant plasmid pET-amastin.Results: The amastin gene of 550bp was acquired by PCR.The recombinant plasmid pET-amastin was identified to be correct.Conclusion: The prokaryotic expression recombinant plasmid of amastin gene of L.donovani has be... |
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| Keywords: | Leishmania donovani amastin prokaryotic expression gene cloning |
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