神经肽Y在海洛因戒断、脱毒及复吸大鼠直肠组织的表达

目的：探讨海洛因戒断、脱毒及复吸期间大鼠直肠内神经肽Y（NPY）的表达。方法：正常雄性SD大鼠随机分为实验组、盐水对照组和正常对照组,实验组又分戒断组、美沙酮脱毒组和复吸组,采用免疫组织化学SABC法及图像分析方法观察各组大鼠直肠NPY的表达。结果：（1）与正常对照组及盐水对照组比较,戒断组及复吸组大鼠直肠内的NPY阳性细胞免疫染色加深;图像分析NPY阳性细胞的细胞计数增多,平均灰度值低于正常及盐水对照组（P〈0.05）;（2）脱毒组与正常及盐水对照组比较,NPY阳性细胞免疫染色、细胞计数及平均灰度值变化差异均无统计学意义（P〉0.05）。结论：海洛因戒断及复吸期间,NPY在直肠黏膜表达增强,用美沙酮脱毒治疗后NPY的表达接近正常,提示NPY可能参与海洛因戒断、脱毒和复吸期间机体的调节过程。

The Expression of NPY in Rat Rectum during Abstinence,Detoxification and Relapse to Heroin

Objective: To explore the expression changes of neuropeptide Y(NPY) in rat rectum during abstinence,detoxication and relapse to heroin.Methods: Normal male SD rats were randomly divided into normal control group(group NC),saline control group(group SC) and experiment groups including abstinence group(group HA),methadone detoxication group(group MD) and heroin relapse group(group HR).Rat rectum tissues of each group were taken and treated with immunohistochemical SABC method.Image analysis was performed.Results: Compared with those in groups NC and SC,the immunostaining was deeper,the cell counts were bigger,and the mean grey degrees were lighter obviously in NPY positive cells in groups HA and HR(P<0.05).Compared with groups NC and SC,no statistical differences were found in immunostaining,cell counting and mean grey degree of NPY positive cells(P>0.05) in group MD.Conclusions: The expression of NPY positive cells increases during abstinence and relapse of heroin in tunica mucosa recti of rats and turned normal after treatment with methadone.It suggests that NPY participate in adjusting process of organism during abstinence,detoxification and relapse of heroin.