| 黄芪多糖对2型糖尿病患者外周血内皮祖细胞体外增殖的影响 |
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| 引用本文： | 吴青,徐寒松,谢晓云,陈文群. 黄芪多糖对2型糖尿病患者外周血内皮祖细胞体外增殖的影响[J]. 贵阳医学院学报, 2011, 36(2): 119-123 |
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| 作者姓名： | 吴青 徐寒松 谢晓云 陈文群 |
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| 作者单位： | 1. 贵阳中医学院第二附属医院内分泌科,贵州贵阳,550003
2. 中南大学湘雅三医院,湖南长沙,410000 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 目的:观察黄芪多糖(Astragalus Polysaccharides,APS)对2型糖尿病(T2DM)患者内皮祖细胞(EPCs)体外增殖的影响.方法:密度梯度离心法获取T2DM患者外周血单个核细胞,在鼠尾胶原包被的培养瓶中培养7 d后鉴定EPCs,MTT检测APS对T2DM患者EPCs增殖的影响.结果:T2DM外周...
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| 关 键 词： | 内皮 血管 细胞培养 黄芪多糖 糖尿病 非胰岛素依赖型 |
Effect of Astragalus Polysaccharides on Proliferation of Endothelial Progenitor Cells from Peripheral Blood of Patients with Type 2 Diabetes |
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| WU Qing,XU Hansong,XIE Xiaoyun,CHEN Wenqun. Effect of Astragalus Polysaccharides on Proliferation of Endothelial Progenitor Cells from Peripheral Blood of Patients with Type 2 Diabetes[J]. Journal of Guiyang Medical College, 2011, 36(2): 119-123 |
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| Authors: | WU Qing XU Hansong XIE Xiaoyun CHEN Wenqun |
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| Affiliation: | 1(1.The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang 550003,Guizhou,China;2.The Third Xiangya Hospital of Centre-south University,Changsha 410000,Hunan,China) |
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| Abstract: | Objective: To investigate the methods of isolating and culturing endothelial progenitor cells(EPCs) from peripheral blood in patients with type 2 diabetes(T2DM),and to observe the effect of astragalus polysaccharides(APS) on proliferation of EPCs.Methods: Total mononuclear cells were isolated with density gradient centrifugation and put into the culture flasks previously coated by rat tail collagen.After 7 days of culture,EPCs were identifyed,and treated with various concentrations of APS for different durations.Proliferation of EPCs was measured by MTT.Results: Most of the cultured cells adhering to the culture flask displayed fibroblast-like and slabstone-like morphology.Endothelial cell specific antigen CD34 and KDR,and progenitor antigen CD133 were expressed.APS could promote the proliferation of EPCs in T2DM in certain ranges of dose and time(P<0.05).Conclusions: The rat tail collagen can be a cheaper replacement of fibronectin for the culture of EPCs from peripheral blood in T2DM.APS could markedly promote the proliferation of EPCs in T2DM. |
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| Keywords: | endothelium,vascular cell culture astragalin polysaccharides diabetes mellitus,non-insulin dependent |
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