| SW626细胞染色体动粒变异对其染色体不稳定的影响 |
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| 引用本文： | 李爽,谭彬,刘学庆,陈雪梅,丁裕斌,余秋波,陈倩,王应雄,何俊琳.SW626细胞染色体动粒变异对其染色体不稳定的影响[J].第三军医大学学报,2011,33(7). |
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| 作者姓名： | 李爽 谭彬 刘学庆 陈雪梅 丁裕斌 余秋波 陈倩 王应雄 何俊琳 |
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| 作者单位： | 重庆医科大学公共卫生学院遗传优生教研室,重庆,400016 |
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| 基金项目： | 国家自然科学基金，重庆市人口与计划生育委员会基金 |
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| 摘 要： | 目的 探讨恶性肿瘤细胞染色体不稳定性的可能机制.方法 采用本实验室建立的kinetochore-NOR同步银染技术,对SW626细胞染色体kinetochore变异进行分析.结果 与正常人外周血细胞相比,SW626细胞染色体kinetochore缺失132(0.89%) vs 26(0.18%)]、kinetochore迟滞复制82(0.55%) vs 14(0.10%)]和kinetochore-NOR融合153(1.03%) vs 51(0.37%)]频率显著升高(P<0.01),而不对称kinetochore频率二者差异性不显著(P>0.05).此外,在某些SW626细胞染色体上还观察到多重kinetochores现象.结论 kinetochore 缺失、kinetochore迟滞复制和kinetochore-NOR融合可能是SW626细胞染色体非整倍性变异起源的诱因之一;染色体多重kinetochores可能是恶性肿瘤细胞染色体结构畸变产生的重要途径之一.
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| 关 键 词： | 染色体不稳定 kinetochore变异 SW626细胞 |
Effect of kinetochore variation on chromosomal instability in SW626 cells |
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| Li Shuang,Tan Bin,Liu Xueqing,Chen Xuemei,Ding Yubin,Yu Qiubo,Chen Qian,Wang Yingxiong,He Junlin.Effect of kinetochore variation on chromosomal instability in SW626 cells[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(7). |
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| Authors: | Li Shuang Tan Bin Liu Xueqing Chen Xuemei Ding Yubin Yu Qiubo Chen Qian Wang Yingxiong He Junlin |
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| Institution: | Li Shuang,Tan Bin,Liu Xueqing,Chen Xuemei,Ding Yubin,Yu Qiubo,Chen Qian,Wang Yingxiong,He Junlin(Department of Genetics and Eugenics,College of Public Health,Chongqing Medical University,Chongqing,400016,China) |
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| Abstract: | Objective To probe possible mechanism of chromosomal instability in tumor.Methods Kinetochore variation was analyzed by an improved method for staining kinetochores of human chromosomes in SW626 cells.Results Compared to normal cells,the frequency of kinetochore loss132(0.89%) vs 26(0.18%)],kinetochore duplication laggard 82(0.55%) vs 14(0.10%)] and kinetochore-NOR fusion 153(1.03%) vs 51(0.37%)] in SW626 cells was significantly higher than those of human normal periphoreal blood cells respectively(P<0.0... |
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| Keywords: | chromosomal instability kinetochore variation SW626 cells |
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