丹参对大鼠肝脏缺血再灌注损伤的抗氧化作用

目的探讨丹参对大鼠缺血再灌注损伤肝脏的抗氧化作用。方法建立大鼠全肝缺血再灌注模型,60只大鼠随机分为三组,每组20只,分别为对照组(A组)、肝缺血/再灌注组(B组)和肝缺血/再灌注加丹参治疗组(C组),分别在肝缺血前、缺血45min、再灌注45min共3个时相点,检测血浆超氧化物歧化酶(SOD)活性、黄嘌呤氧化酶(XO)活性、丙二醛(MDA)浓度以及血清谷丙转氨酶(ALT)活性。结果肝缺血/再灌注组,血浆XO、MDA及ALT显著高于对照组、SOD明显低于对照组(P<0.05和<0.01)。而丹参治疗组与缺血/再灌注组比较,上述指标均有显著性差异(P<0.05和<0.01)。结论丹参可通过降低氧自由基水平(增强SOD活性、减弱XO活性),拮抗脂质过氧化反应(降低MDA浓度),从而减轻肝脏缺血再灌注损伤。

Anti - oxidation role of salvia miltiorrhiza against hepatic ischemia- reperfusion injury in rats

Objective To investigate the anti-oxidation role of salvia miltiorrhiza(SM) against hepatic ischemia-reperfusion injury in rats.Methods 60 cats were selected and divided into three groups randomly(n=20),sham operated control group(A),ischemia-reperfusion group(B) and ischemia-reperfusion SM group(C).Levels of malondialdehyde(MDA),superoxide dismutase(SOD),xanthine oxidase(XO) and alanine aminotransferase(ALT) were measured before ischemia,45 minutes after ischemia and 45 minutes after reperfusion respectively.Results Both the levels of XO and MDA were increased at 45 minutes after reperfusion,while SOD decreased(P<0.05 and P<0.01).Differences of each items between group B and C were significant(P<0.05 and P<0.01).Conclusion SM may attenuate hepatic ischemia-reperfusion injury by dropping oxygen free radicals level(raising SOD activity and dropping XO activity) and antagonizing lipid peroxidation(reducing MDA content).