猪视网膜色素上皮细胞间连接与通讯功能的关系

目的：探讨体外培养的猪视网膜色素上皮(retinal pigment epithelial,RPE)细胞间连接与通讯功能的关系。 方法：用5，6-碳氧荧光素醋酸盐进行细胞染色，光淬灭某些细胞中的荧光分子，激光扫描共聚焦显微镜观察不同连接状态的RPE细胞内的荧光恢复速率，评价缝隙连接的通讯功能。 结果：光漂白后的ＲＰＥ细胞中的荧光强度得以恢复，与之紧密相连的细胞则有荧光下降，分别与1, 2, 3个细胞相连的每分钟荧光恢复速率依次是1.997±0.665，4.378±0.811，8.736±2.084。 结论：体外培养的猪RPE细胞缝隙连接通讯功能的强弱与细胞的连接状况有关。 (中华眼底病杂志,1998,14:41-42)

Evaluation of gap junction mediated intercellular communication in cultured porcine retinal pigment epithelial cells

Purpose:To evaluate the function of gap junction-mediated intercellular communication in cultured cells of retinal pigment epithelial(RPE) cells from porcine eyes. Methods:The cultured RPE cells were previously stained by a fluorescent probe 5, 6-carboxy fluorescein diacetate (CFDA) ,and then photobleach the fluorescent molecule in chosed cells. Using laser scanning confocalmicroscope (LSCM)to observe fluorescence recovery rate of the RPE cells which located in different condition. The function of gap junction communication was evaluated according to the fluorescence recoveryrate. Results:The fluorescence recovered after photobleached and the fluorescent density of cells which touching to them descend. The recovery rate per minnte of the cells which the cell number it adjacent to was 1,2 and 3 respectively was 1. 997±0. 665, 4. 378±0. 811 and 8. 736±2. 084. Conclusion:The cultured porcine RPE cells have the function of gap junction communication,and its function proportion is associate to its adjoining cells number. （Chin J Ocul Fundus Dis,1996,12: 41-42）