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Molecular and antigenic characterization of rabbit hemorrhagic disease virus isolated in Cuba indicates a distinct antigenic subtype |
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| Authors: | O Farnós D Rodríguez O Valdés M Chiong F Parra J R Toledo E Fernández R Lleonart M Suárez |
| Institution: | (1) Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba;(2) Centro Nacional de Epizootiología, Diagnóstico e Investigación, La Habana, Cuba;(3) Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, Oviedo, Spain |
| Abstract: | Summary. Phylogenetic analyses conducted on isolates of rabbit hemorrhagic disease virus (RHDV) from throughout the world have shown well-defined genogroups comprising representative strains of the virus and antigenic variants. In this work, we have isolated and characterized RHDV from the major epizootic that occurred in Cuba in 2004–2005. Sequence analysis of the capsid protein gene and antigenic characterization of this strain has allowed its inclusion as a member of the distinct RHDVa subtype. We also found that specific antibodies directed against RHDV reference strains bound to the Cuban isolate in a competition ELISA and inhibited virus hemagglutination in vitro. This is the second report on the molecular characterization of RHDVa circulating in the American region. First three authors contributed equally to this work. |
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