| 反相高效液相色谱法测定梅花点舌丸中酯蟾毒配基、华蟾毒配基的含量 |
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| 引用本文： | 李亚芳,胡晓炜. 反相高效液相色谱法测定梅花点舌丸中酯蟾毒配基、华蟾毒配基的含量[J]. 中国药房, 2002, 13(6): 362-363 |
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| 作者姓名： | 李亚芳 胡晓炜 |
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| 作者单位： | 1. 杭州市第一人民医院,杭州市,310006
2. 杭州市药品检验所,杭州市,310014 |
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| 摘 要： | 目的 :建立测定梅花点舌丸中酯蟾毒配基、华蟾毒配基的反相高效液相色谱法。方法 :采用KromasilC18 柱 ,流动相为乙腈∶0 5 %KH2PO4 溶液 (50∶50) ,检测波长为296nm。结果 :该方法线形关系良好 ,酯蟾毒配基的平均加样回收率为97 40 % ,RSD为2 2 % ;华蟾毒配基的平均加样回收率为97 78 % ,RSD为2 6 %。结论 :方法简便可靠 ,分离度较好 ,可用于梅花点舌丸的质量控制。
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| 关 键 词： | 梅花点舌丸 酯蟾毒配基 华蟾毒配基 反相高效液相色谱法 含量测定 |
| 文章编号： | 1001-0408(2002)06-0362-02 |
| 修稿时间： | 2001-07-06 |
Determination of Resibufogenin and Cinobufagin in Meihuadianshe Pills by RP- HPLC |
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| LI Yafang. Determination of Resibufogenin and Cinobufagin in Meihuadianshe Pills by RP- HPLC[J]. China Pharmacy, 2002, 13(6): 362-363 |
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| Authors: | LI Yafang |
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| Abstract: | OBJECTIVE:To establish a RP-HPLC method for determination of the contents of resibufogenin and cinobufagin in Meihuadianshe pills METHODS:The condition of HPLC was Kromasil C18 column,acetonitrile-0 5%KH2PO4(50∶50) as a mobile phase and detecting wavelength 296nm RESULTS:This method showed good linearity The average recoveries of resibufogenin and cinobufagin were 97 40% with a RSD of 2 2% and 97 78% with a RSD of 2 6% respectively CONCLUSION:The method is simple,reliable and satisfactory in resolution It can be used in quality control of Meihuadianshe pills |
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| Keywords: | Meihuadianshe pill resibufogenin cinobufagin RP-HPLC determination of content |
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