青葙子提取物降血糖活性的研究

 目的研究青葙子提取物对四氧嘧啶糖尿病小鼠降血糖作用。方法采用体积分数95%乙醇和水连续浸提青葙子获得醇提(A)和水提浸膏(B)。醇提浸膏用石油醚、醋酸乙酯、正丁醇连续萃取,获得不同部分。水提物采用3倍乙醇沉淀分为醇溶部分和粗多糖。尾静脉注射四氧嘧啶建立小鼠糖尿病模型,给予青葙子提取物灌胃,1 g·kg^-1或800 mg·kg^-1,连续3周, 测定血糖、血清中胰岛素含量及饮水量和耗食量,测定小鼠肝、肾、脾和胰腺重量指数。结果青葙子醇提物和水提物均有一定降血糖活性,其中醇提物的正丁醇部分(A-c)和水提物中粗多糖部分(B-b)具有显著降血糖活性,粗多糖部分具有明显促进胰岛素分泌作用,效果优于格列本脲(2 mg·kg^-1)。A-c和B-b能使四氧嘧啶引起的糖尿病小鼠肾脏和肝脏的肿胀恢复接近正常。结论青葙子提取物时四氧嘧啶糖尿病小鼠具有降血糖作用。

Hypoglycemic effect of Celosia argentea fractions in alloxan-induced diabetic mice

OBJECTIVE To evaluate the hypoglycemic effects of alcohol extract, water extract and their subfractions from the seeds of Celosia argentea L. in alloxan-diabetic mice.METHODS The seeds were extracted with ethanol and water successively. The ethanol-extract (A) was partitioned with petholeum ether, ethyl acetate and n-butanol to yield three fractions (A-a, A-b, A-c) and the water-extract (B) was precipitated with 3 times volume ethanol to divided into two parts (B-a, B-b). The hypoglycemic effects were investigated in alloxan-induced diabetic mice after oral administration of 1.0 g·kg^-1 or 800 mg·kg^-1 body weight for two or three weeks. The blood glucose, plasma insulin, the consumption of water and food, weight index of liver, kidney,spleen and pancreas were observed. RESULTS Both of A and B exhibited hypoglycemic effects. Among these fractions, A-c and B-b markedly reduced blood glucose level in diabetic mice compared with control group (P< 0.05), inhibited significantly the swelling of liver and kidney in diabetic mice induced by alloxan. B-b increased significantly plasma insulin levels in diabetic mice. A-a and B-b decreased the intake of water and food. The antidiabetic activities of A-c and B-b were stronger than that of 2 mg·kg^-1 glibenclamide. CONCLUSION The n-butanol fraction and crude polysaccharides of Celosia argentea L. possess significant hypoglycemic activities.