慢性阻塞性肺病患者外周血树突状细胞的检测及其临床意义

目的 观察慢性阻寨性肺病(COPD)患者急性加重期与稳定期外周血树突状细胞的变化，探讨外周血树突状细胞(DC)与其病情的关系。方法 共41例COPD患者进入研究，分别在其急性加重期与稳定期抽静脉血，分离并进行免疫组织化学染色，观察计数树突状细胞，同时检测T淋巴细胞亚群CD4、CD8，并行血气分析及肺动脉压测定进行比较。结果 稳定期外周血树突状细胞明显高于急性加重期(P＜0．01)，并且病情严重程度与外周血树突状细胞计数呈负相关。结论 COPD急性加重期均有不同程度外周血树突状细胞减少，在一定程度上反映病人免疫功能状况，检测外周血树突状细胞有助于了解病情、判断预后、指导防治。

Determination and clinical sense of peripheral dendritic cell in chronic respiratory failure patients

Objective To study the changes of peripheral dendritic cell (DC) in acute attack and catabasis of chronic obstructive pulmonary disease(COPD) and the correlation with severity. Methods 41 patients with COPD entered the study. Venous blood samples were respectively obtained in attack and catabasis, then separated and stained with immunohistochemistry. DC were counted and CD4, CD8 were detected, blood gas analysis and the pressure of pulmonary artery were adopted. Results The number of DC in catabasis was significantly higher than in acute attack (P<0.01), and the severity is negative correlative with the number of peripheral DC. Conclusions The peripheral DC in actue stage is decreased differently. Early inspecting peripheral DC helped to observe the case and Immune function, estimate prognosis and direct threapy.