庚型肝炎病毒E2蛋白在转基因小鼠细胞内的定位及致病性研究

目的 :在已建成庚型肝炎病毒 (HGV)转基因小鼠模型的基础上 ,研究 HGV包膜蛋白 E2在转基因小鼠体内的表达及定位 ,并探讨 HGV在转基因小鼠体内是否引起病理学改变。 方法 :取小鼠的器官组织用免疫组织化学方法 ,以 HGV E2蛋白的单克隆抗体对 HGV包膜蛋白 E2的表达进行分析和定位。通过血清 AL T检测及常规病理学技术观察转基因小鼠不同器官的病理学变化。 结果 :免疫组织化学结果表明 ,HGV E2主要表达于转基因小鼠肝细胞膜 ,少量表达于胞质内 ;肾脏、心脏、肺及脾脏均未呈现 E2蛋白的阳性反应。组织病理学检查显示转基因小鼠的肝细胞出现水样变性、脂肪变性及淋巴细胞浸润等轻度炎性反应 ,这些变化不随年龄增长而加重 ;其他组织的病理学检查无异常。转基因小鼠血清转氨酶 AL T与对照小鼠无明显差异。 结论 :HGV包膜蛋白 E2的表达具有相对的嗜肝性 ,主要分布在转基因小鼠的肝细胞膜 ,并可引起轻度肝细胞损伤。

Location and pathogenicity of HGV E2 protein in transgenic mice

Objective: To observe the expression and location of HGV envelope protein E2, and investigate the pathogenicity of HGV in transgenic mice. Methods: Immunohistochemistry technique was explored to analyze the expression and sublocation of HGV E2 protein. Serum ALT and histopathology slices were performed to study the pathogenicity of HGV transgenic mice. Results: Immunohistochemistry data suggested that HGV E2 protein expressed mainly on hepatocyte membrance, with few in hepatocytoplasm. There was no positive result of E2 in kidney, heart, lung and spleen of the transgenic mice. The pathological observation indicated that the hydrogenic degeneration, steaosis and lymphoid cell infiltration appeared in liver, instead of other organ or other tissues. However, serum ALT had no significant difference between the normal and transgenic mice. Conclusion: HGV E2 protein is expressed on hepatocyte membrance, HGV genes can cause slight damage in liver of the transgenic mouse. [