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中国安徽地区肝癌组织中HBV基因型的分布研究
引用本文:唐克明,高伏圣,张春林,刘辉.中国安徽地区肝癌组织中HBV基因型的分布研究[J].中国现代医学杂志,2008,18(12).
作者姓名:唐克明  高伏圣  张春林  刘辉
作者单位:1. 海军安庆医院,安徽,安庆,246003
2. 第二军医大学东方肝胆外科医院,肝外三科,上海,200438
摘    要:目的研究中国安徽地区肝癌组织中HBV基因型的分布状况。方法应用基因型特异性引物PCR法对96份肝细胞癌组织和90份HBV携带者血清进行HBV基因型检测。结果在65份分型的肝癌组织标本中,B基因型23份(35.4%),C基因型28份(43.1%),B+C混合基因型8例(12.3%),B+D混合基因型6例(9.2%)。在90份HBV携带者血清中,B基因型39份(43.3%),C基因型35份(38.9%),B+C混合基因型12例(13.3%),B+D混合基因型4例(4.4%)。C基因型在肝癌组织中占优势,而HBV携带者血清以B基因型为主。结论中国安徽地区肝细胞癌组织中存在基因型B、C及混合基因型B+C与B+D,以C基因型为主,其次为B基因型。

关 键 词:肝细胞癌  HBV基因型  中国  安徽  地区  肝癌组织  基因型检测  分布研究  hepatocellular  carcinoma  patients  genotype  hepatitis  B  virus  存在  优势  混合  组织标本  分型  结果  血清  携带者  肝细胞癌组织  型特异性引物

Study on hepatitis B virus genotype in patients with hepatocellular carcinoma
TANG Ke-ming,GAO Fu-sheng,ZHANG Chun-lin,LIU Hui.Study on hepatitis B virus genotype in patients with hepatocellular carcinoma[J].China Journal of Modern Medicine,2008,18(12).
Authors:TANG Ke-ming  GAO Fu-sheng  ZHANG Chun-lin  LIU Hui
Institution:TANG Ke-ming1,GAO Fu-sheng1,ZHANG Chun-lin1,LIU Hui2 (1.The Navy Hospital in Anqing,Anqing,Anhui 246003,P.R.China,2.Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200438,P.R.China)
Abstract:Objective] To investigate the distribution of HBV genotype of hepatocellular carcinoma (HCC) patients in Anhui provinces. Methods] HBV genotypes were determined with nested-PCR by using type-specific primers in 96 HCC tumour tissues, control samples were done in 90 HBV carriers. Results] Of the 96 HCC patients, the genotypes of HBV genomic DNA of 65 HCC patients were successfully determined by PCR with type-specific primers. 23 (35.4%) were genotype B, 28 (43.1%) were genotype C, 8 (12.3%) were genotype ...
Keywords:hepatocellular carcinoma  HBV genotype  
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