针药结合对脾虚证大鼠心功能的影响

目的:探讨脾虚证心功能的改变及针刺结合西洋参汤对脾虚证心功能改变的影响。方法:Wistar大鼠48只,随机分为正常对照组、模型组、西洋参组及针药结合组,每组各12只。模型组、西洋参组、针药结合组用偏食法建立脾虚证动物模型。造模成功后,西洋参组给予西洋参汤灌胃;针药结合组针刺双侧"足三里"、双侧"三阴交",平补平泻法捻转行针1min,留针20min,每日1次,并给予西洋参汤1ml/100g灌胃。治疗10d后,测定左室舒张末期压(LVEDP)、左室内压(LVP)、心率(HR)、收缩压(SBP)、舒张压(DBP)和心输出量(CO)。结果:与正常对照组比较,针药结合组、西洋参组的HR、SBP、DBP、CO均有所下降,但差异无统计学意义(P>0.05),3组与模型组比较,差异有统计学意义(P<0.05)。结论:脾虚动物HR、SBP、DBP、CO均下降,西洋参和针药结合对其具有调节作用,使其接近正常水平,该结果为临床治疗脾虚证心功能改变提供了依据。

Effect of Acupuncture Combined with American Ginseng on Cardiac Function in Rats with Spleen-deficiency Syndrome

Objective To observe the changes of cardiac function of spleen-deficiency syndrome after joint administration of acupuncture and medication. Methods A total of 48 Wistar rats were randomized into normal control, model, medication (American ginseng, AG) and acupuncture + AG groups. Spleen-deficiency syndrome model was established by monophagia method. Rats of medication group were fed with AG decoction (1 ml/100 g), once daily for 10 days. Acupuncture stimulation (uniform reinforcing and reducing method) was applied to bilateral "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) for 1 min (with the needles retained for 20 min), once daily for 10 days. Then, all the rats anesthetized underwent right common carotid artery-left-cardiac catheter and right femoral arterial catheter surgery to accept detection of the left ventricular end diastolic pressure (LVEDP), left ventricular pressure (LVP), heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP) and cardiac output (CO). Results Compared with normal control group, the levels of SBP, DBP, HR and CO of model group were significantly lower (P<0. 05). In comparison with model group, SBP, DBP, HR and CO of medication and acupuncture + AG groups were markedly higher (P<0. 05). No significant differences were found between medication and acupuncture + AG groups in SBP, DBP, HR, LVEDP, LVP and CO levels (P>0. 05). Conclusion Administration of both acupuncture + AG and AG can effectively improve spleen-deficiency syndrome rats' cardiac function.