| 大鼠额叶损伤后细胞凋亡及iNOS的表达变化 |
| |
| 引用本文： | 何志贤,董玉林,张志军,郁晓燕,倪衡建.大鼠额叶损伤后细胞凋亡及iNOS的表达变化[J].苏州大学学报(自然科学版),2008,28(5). |
| |
| 作者姓名： | 何志贤 董玉林 张志军 郁晓燕 倪衡建 |
| |
| 作者单位： | [1]南通大学医学院外总教研室,江苏南通226001 [2]南通大学医学院人体解剖学教研室,江苏南通226001 |
| |
| 基金项目： | 江苏省高校自然科学基金指导计划 |
| |
| 摘 要： | 目的探讨大鼠额叶锐器损伤后细胞凋亡的变化规律及可能机制。方法采用大鼠额叶锐器损伤模型,用TUNEL法检测细胞凋亡,用RT-PCR和Western blot检测诱导性一氧化氮合酶（iNOS）基因和蛋白的表达变化。结果凋亡细胞在损伤后3h即可发现,24h达到高峰,随后逐渐减少;伤后3hiNOS基因和蛋白的表达开始上升,24h达到高峰,以后也逐渐下降。结论大鼠额叶锐器伤后存在细胞凋亡,凋亡细胞数量的变化与伤后时程有关,iNOS表达增加可能是影响细胞凋亡的重要因素。
|
| 关 键 词： | 额叶损伤 凋亡 诱导性一氧化氮合酶 大鼠 |
Changes of Cell Apoptosis and Expression of iNOS after Frontal Injury in Rats |
| |
| HE Zhi-xian,DONG Yu-lin,ZHANG Zhi-jun,YU Xiao-yan,NI Heng-jian.Changes of Cell Apoptosis and Expression of iNOS after Frontal Injury in Rats[J].Suzhou University Journal of Medical Science,2008,28(5). |
| |
| Authors: | HE Zhi-xian DONG Yu-lin ZHANG Zhi-jun YU Xiao-yan NI Heng-jian |
| |
| Institution: | HE Zhi-xian1,DONG Yu-lin2,ZHANG Zhi-jun2,YU Xiao-yan2,NI Heng-jian2 (1.Dept of Surgery,2.Dept of Anatomy,Medical School of Nantong University,Jiangsu Nantong 226001,China) |
| |
| Abstract: | Objective To investigate the alterations and possible mechanism of cell apoptosis after frontal sharp injury in rats. Methods Using frontal sharp cortex injury model, RT-PCR, western-blot and TUNEL methods were used to determine iNOS mRNA, iNOS protein and apoptotic cells respec- tively. Results The apoptotic cells were found as early as 3 hours after injury and reached a peak 72 hours, and then decreased in the following time. The iNOS mRNA and iNOS protein increased 3 hours after frontal injury, peaked at... |
| |
| Keywords: | frontal injury apoptosis inducible nitric oxide synthase rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
