腹透液对大鼠腹膜间皮细胞水通道蛋白1表达的影响

目的探讨腹透液对大鼠腹膜间皮细胞水通道蛋白1(AQP1)表达的影响。方法将体外培养的大鼠腹膜间皮细胞分成4组,分别用含10%胎牛血清的1640培养液(阴性对照组)和3种不同腹透液(分别含4.25%葡萄糖、4.25%甘露醇、1.5%葡萄糖)刺激3h,采用间接免疫荧光标记法、流式细胞仪检测上述不同腹透液刺激后AQP1的表达。结果4.25%葡萄糖腹透液组和4.25%甘露醇腹透液组腹膜间皮细胞AQP1表达显著大于阴性对照组和1.5%葡萄糖腹透液组(P<0.01);4.25%葡萄糖腹透液组腹膜间皮细胞AQP1表达显著大于4.25%甘露醇腹透液组(P<0.01);1.5%葡萄糖腹透液组腹膜间皮细胞AQP1表达与阴性对照组相比无显著性差异(P>0.05)。结论高糖腹透液可以促进腹膜间皮细胞表面AQP1的表达,糖本身或糖降解产物可能是促进其表达的独立因素。

Effects of peritoneal dialysate on the expression of aquaporin-1 in rat peritoneal mesothelial cells

Objective To discuss the effects of peritoneal dialysate (PDS) on the expression of aquaporin-1 in rat peritoneal mesothelial cells (RPMCs). Methods The in vitro cultured RPMCs were divided randomly into 4 groups, and stimulated respectively with 1640 culture medium containing 10% fetal calf serum (negative control), and three kinds of PDS (containing respectively 4.25% glucose, 4.25% mannitol or 1.5% glucose) for 3 hours. Indirect immunofluorescence assay (IFA) and flow cytometry (FCM)were employed to detect the expression of aquaporin-1 in RPMCs stimulated by different kind of PDS as above. Results Compared with negative control and the group with PDS containing 1.5% glucose, the expression of aquaporin-1 on the mesothelial cells of the group with PDS containing 4.25% glucose and that containing 4.25% mannitol was significantly up-regulated (P<0.01). Higher expression of aquaporin-1 was found in the group with PDS containing 4.25% glucose compared with that in the group with PDS containing 4.25% mannitol (P<0.01). No significant differences on the expressions of aquaporin-1 were found between the negative control and the group with PDS containing 1.5% glucose (P>0.05). Conclusion PDS with higher osmotic pressure (OP) can enhance the expression of aquaporin-1 in RPMCs. For PDS with same OP (PDS containing 4.25% glucose or PDS containing 4.25% mannitol), those containing glucose can enhance the expression of aquaporin-1 more effectively than that PDS containing mannitol. This result indicates that glucose or its degradation products may be the independent factors in enhancing the expression of aquaporin-1 of RPMCs.