去势前后大鼠腹叶前列腺显微结构的动态变化

目的：探讨去势后大鼠前列腺显微结构变化规律及机制。方法：90只大鼠分为9组，于去势后不同时间光镜、电镜下观察前列腺改变，用图像分析系统计算腺体面积与间质面积比值（G），管腔分泌物与管腔面积比值（S），腺体高度（H）及腺体横截面细胞数（N）。采用TUNEL法检测凋亡。免疫组化检测PCNA，计算凋亡指数（AI）和增殖指数（PI）。结果：去势后G、S、H下降，N减少。出现脂肪变、凋亡及坏死，增殖下降。结论：去势后前列腺内细胞萎缩、分泌物减少、坏死、AI升高和PI降低共同导致前列腺缩小。

The micro-morphological changes of rats' ventral prostates following castration

Objective To reveal the morphologic changes of rats' prostates after castration, and to explore the possible mechanism that causes these morphologic changes. Methods 90 male adult rats were randomized into 9 groups (10 per group), one group as the control, while the others were sacrificed 1, 2, 3, 5, 7, 10, 14, 21 days post castration. Prostates were harvested, then fixed, embedded, sectioned, and observed by light microscope and electric telemicroscope. Furthermore, sections of the prostates were detected for apoptosis by TUNEL and PCNA by immunohistochemistry. Sections stained by HE were applied to computer-based imaging analysis system in order to count the area of glands, stroma and the width of the epithelium separately. Results Prostatic glands were atrophied, and the secretion of glands was diminished post castration. Apoptosis and necrosis occurred in the epithelia as well as in the stromal cells and the proliferation index was decreased. Conclusions Weight and volume loss of the prostates after castration was due to the loss and atrophy of the prostate cells as well as the decrease of secretion. Apoptosis and proliferation might play a role in the atrophy of the prostate.