| 大鼠肝脏缺血再灌注损伤c-fos表达及预处理的保护作用 |
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| 引用本文： | 张效杰,贾晋斌,鲍民生,李正中. 大鼠肝脏缺血再灌注损伤c-fos表达及预处理的保护作用[J]. 中华肝脏病杂志, 1999, 0(Z1) |
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| 作者姓名： | 张效杰 贾晋斌 鲍民生 李正中 |
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| 作者单位： | 山西医科大学第一医院肝胆外科!030001(张效杰，贾晋斌，鲍民生)，山西医科大学肝病研究所(李正中) |
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| 基金项目： | 山西青年科学基金!97025 |
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| 摘 要： | 目的探讨预处理（preconditioningPc）对大鼠肝脏缺血再灌注（ischemia／reperfusion，I／R）损伤的影响及其机制。方法制备大鼠肝脏原位I／R损伤的模型，采用免疫组织化学技术结合图像分析方法定量检测原癌基因c-fos表达的情况和肝组织脂质过氧化产物丙二醛（MDA）的变化。结果I／R损伤早期可引起损伤区肝细胞核内原癌基因c－fos的大量表达；PC明显减少了c－fos表达的细胞数量以及减轻肝脏脂质过氧化的程度。结论PC对大鼠肝脏I／R损伤有明显的保护作用，可能的机制之一是抑制肝I／R损伤后原癌基因c-fos的表达和灭活自由基减少脂质过氧化物的生成。
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| 关 键 词： | 肝脏 缺血再灌注 基因，fos 免疫组织化学 预处理 |
Expression of c-fos following ischemia/reperfusion injury and preconditioning protection in rat liver |
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| ZHANG Xiaojie, JIA Jinbin, BAO Minsheng et al. Expression of c-fos following ischemia/reperfusion injury and preconditioning protection in rat liver[J]. Chinese journal of hepatology, 1999, 0(Z1) |
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| Authors: | ZHANG Xiaojie JIA Jinbin BAO Minsheng et al |
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| Affiliation: | ZHANG Xiaojie, JIA Jinbin, BAO Minsheng et al. Department of Surgery,First Affiliated Hospital,Shanxi Medical University,Taiyuan 030001 [ |
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| Abstract: | Objective To investigate the expression of the immediate early gene c-fos following ischemia/reperfusion injury and the effect of preconditioning on I/R injury in the rat liver. Methods On the role of in situ hepatic I/R injury of the rats, changes of expression of c-fos, which has been hypothesized to be tile key factor in determining tissue regeneration following I/R injury, were examined in the rat liver by immunocy-tochemical technique combined with image analysis method, MDA activity in liver tissues were observed. Results Hepatic I/R injury induced c-fos expression within damaged regions of the rat liver at early phase(1-3h) post reper fusion; PC inhibited c-fos expression and attenuated formation of MDA(P<0.01). Conclusion Our results suggest that PC can protect liver from I/R injury; one of the POSSible mechanisms is to inhibit c-fos expression, inactivate oxygen, free radicals and reduce formation of MDA. [ |
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| Keywords: | : Liver Ischemia / reper fusion (I / R ) Gene fos Immunohistochemistry Precondi tioning |
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