华东地区散发性戊型肝炎病毒系统进化分析

目的 了解华东地区散发性戊型肝炎病毒(HEV)的系统进化特征.方法 2005-2008年收集华东地区14家二级或三级医院413份散发戊型肝炎患者血清,应用巢式RT-PCR方法检测HEVRNA并测序.参照GenBank相关序列,分析各序列之间的同源性,并构建进化树.结果 413例患者的男女性别比为1.75:1,40～69岁患者居多(61.5%).413份患者血清有140份分离出HEV RNA,阳性分离率为34%.所有分离的140份病毒株均属于HEV Ⅳ型,与Ⅰ、Ⅱ、Ⅲ和Ⅳ型参考病毒株的核苷酸同源性分别为77.9%～88.3%、80.8%～90.6%、73.4%～85.2%和91.0%～95.4%.结论 基因Ⅳ型HEV是引起华东地区散发性戊型肝炎的优势病毒株,其起源和进化等问题仍需进一步研究.

Phylogenetic analysis of sporadic hepatitis E virus in Eastern China

Objective To understand phylogenetic characteristics of sporadic hepatitis E virus(HEV)in eastern China.Methods Four hundred and thirteen sera were collected from sporadic hepatitis E cases in 14 second-or first-class hospitals in Eastern China from 2005 to 2008 and detected with a nested RT-PCR assay.Partial nucleotide sequences of the HEV isolates were determined for phylogenetic analysis with propotype sequences in the GenBank.Results The male-to-female sex ratio of the patients was 1.75:1 with 61.5% of them aged 40-69 years old.HEV RNA was detected in 140 out of 413(34%)serum samples.Phylogenetic analysis revealed that all the 140 HEV isolates belonged to genotype Ⅳ,sharing 77.9%-88.3%,80.896-90.6%,73.4%-85.2% and 91.0%-95.4% nucleotide sequence identities with prototype Ⅰ,Ⅱ,Ⅲ and Ⅳ HEV isolates respectively.Conclusion It was evident that genotype Ⅳ HEV served as the main causative agent of sporadic HEV infection in Eastern China.However the viral origin and evolution needs further clarification.