儿童哮喘与幽门螺杆菌感染的相关性研究

目的通过对比分析哮喘患儿与健康儿童幽门螺旋杆菌（Hp）14C感染率以及血浆中白细胞介素-1b（IL-1b）含量的关系,分析探究Hp感染对儿童哮喘的作用机理,找出更佳防治儿童哮喘的对策方法。方法采用分层抽样法随机抽取2010年8月~2012年8月在我院住院治疗的50例哮喘患儿（哮喘组）、50例非哮喘Hp感染患儿（Hp感染正常组）、50例哮喘且Hp感染患儿（Hp感染＋哮喘组）和50例健康儿童（正常组）为受试对象。利用14C尿素呼气检测仪检测Hp感染阳性率,ELISA试剂盒检测血浆中白细胞介素-1b（IL-1b）含量。结果 1哮喘儿童的14C尿素呼气检测阳性率为34.0%（哮喘组）和50.0%（Hp感染＋哮喘组）,明显大于健康对照儿童的4.0%（P〈0.05）,且哮喘和Hp感染正线性相关（R=0.30,P〈0.05）;2Hp菌株感染后的儿童14C尿素呼气检测阳性率明显高于未感染儿童（P〈0.05）,且哮喘和Hp阳性感染概率呈现正线性关系（R=0.47,P〈0.05）。3四组患儿IL-1b都满足正态分布,其中哮喘儿童的IL-1b浓度为（3.68±0.12）ng/m L,低于健康对照儿童的（0.61±0.04）ng/m L,组间对比具有统计学意义（P〈0.05）;Hp感染＋哮喘组儿童IL-1b浓度为（4.77±0.09）ng/m L,明显高于哮喘组儿童的（3.68±0.12）ng/m L,组间对比差异明显（P〈0.05）。结论哮喘儿童与无哮喘儿童幽门螺旋杆菌（Hp）感染率和血浆中白细胞介素-1b（IL-1b）含量有显著差异,这为哮喘患儿诊断提供了监测依据,在儿童哮喘疾病的治疗过程中有较大的应用价值。

A study on relationship between childhood asthma and infection helicobacter pylori

Objective By analyzing children with asthma and healthy children with helicobacter pylori （Hp） infection rate of 14C and the serum levels of interleukin -lb （IL-lb） analysis of the content to explore the relationship between the mechanism of asthma in children infected with Hp and to find a better method for prevention of asthma in children. Methods Stratified sampling randomly from August 2010 to August 2012 in our hospital treatment of 50 cases of chil- dren with asthma （asthma group） and 50 cases of children with asthma Hp infection （Hp infection normal group） and 50 cases of children with asthma and Hp infection （lip infection＋asthma group） and 50 cases health children （normal group） as the subjects. Used 14C urea breath detector to detect Hp infection positive rate, ELISA kits in plasma interleukin lb （IL-1 b） content. Results （1） 1% urea breath test positive rate of asthma children was 34.0% （asthma group） and 50.0% （Hp infection＋asthma group）, which was significantly greater than healthy controls 4.0% of the children, （P〈0.05）, and asthma and Hp infection was linear correlation （R=0.30, P〈0.05）; （2） Hp strains infection after 1% urea breath test positive rate was significantly higher than those uninfected children （P〈0.05）, and asthma and Hp positive probability of infection showed positive linear relationship （R=0.47, P〈0.05）. （3） The four groups of children with IL-lb could meet the normal distribution and the concentration of IL-lb for asthma children was （3.68±0.12） ng/mL, lower than that in the healthy control children （0.61±0.04） ng/mL. Comparison between groups had statistical significance （P〈0.05）; Hp infection ＋concentration of IL-lb for children asthma group was （4.77±0.09） ng/mL, significantly higher than that of asthma group children （3.68±0.12） ng/mL. The comparison between the two groups had obvious difference （P〈0.05）. Conclusion Helicobacter pylori （Hp） infection and the serum l