子宫颈癌组织中COX-2与VEGF的表达及相关性研究

目的:研究环氧合酶-2(Cyclooxygenase,COX-2)及血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)在子宫颈癌及宫颈上皮内瘤样病变Ⅱ、Ⅲ组织中的表达,以探讨COX-2的表达与子宫颈癌发生、发展的关系以及与VEGF之间的相关性。方法:应用逆转录聚合酶链反应(RT-PCR)技术,检测42例子宫颈癌,10例宫颈上皮内瘤样病变Ⅱ、Ⅲ,10例正常宫颈中COX-2 mRNA和VEGF mRNA的表达。结果:COX-2 mRNA,VEGF mRNA在正常、宫颈上皮内瘤样病变Ⅱ、Ⅲ组织及子宫颈癌中的相对含量分别为8.86±19.12、55.76±48.46、132.64±56.99和15.23±24.86、57.30±49.40、142.31±59.45。子宫颈癌及上皮内瘤样病变Ⅱ、Ⅲ组织中COX-2 mRNA,VEGF mRNA的相对含量显著高于正常宫颈组织,差异有统计学意义(P<0.01),子宫颈癌COX-2 mRNA,VEGF mRNA的相对含量高于上皮内瘤样病变Ⅱ、Ⅲ组织,差异有统计学意义(P<0.01)。且COX-2的表达与VEGF的表达呈正相关(r=0.959,P<0.01)。结论:COX-2、VEGF在子宫颈癌组织中高表达,与其发生、发展有密切的关系,VEGF可能是COX-2促进子宫颈癌形成、发展的主要机制之一。

The study on expressions and correlationship of COX-2 and VEGF in cervical cancer

Objective:To investigate the relationship between the expressions of COX-2 mRNA and VEGF mRNA in carcinogenesis and development of the cervical cancer.Methods:Quantitive reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the levels of COX-2 mRNA and VEGF mRNA in the following groups: 10 cases with normal cervical tissue,10 cases with atypical hyperplasia cervical tissue and 42 cases with cervical cancer.Results:The relative content of COX-2 mRNA and VEGF mRNA in normal cervical tiss...