小白菊内酯对结肠癌细胞凋亡的影响及其机制研究 |
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引用本文: | 刘继洪,潘月龙,姚航平. 小白菊内酯对结肠癌细胞凋亡的影响及其机制研究[J]. 中国基层医药, 2009, 16(7): 1550-1551. DOI: 10.3760/cma.j.issn.1008-6706.2009.09.007 |
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作者姓名: | 刘继洪 潘月龙 姚航平 |
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作者单位: | 杭州市第一人民医院肿瘤科,浙江省杭州,310006;浙江大学医学院附属第一医院传染病研究所; |
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摘 要: | Objective To investigate the effect of parthenolide (PN) on human colon cancer cells in vitro and its mechanism. Methods Human colon cancer cell line HT-29 was cultured and treated with PN at the concentra-tions of 10 ,50 ,100 ,200,500 ,1 000 μmol/L for 48 hours. Then the apoptosis was detected by flow cytometry. The ex-pression of NF-Κb p50 and COX-2 proteins were analyzed by Western blot and the concentration of PGE2 was meas-ured with ELISA kits. Results PN significantly induced cell apoptosis after 48 h in a dose-dependent manner. The expression of NF-Κb p50 and COX-2 proteins were down-regulated by the PN, and the concentrations of PGE2 were al-so declined. Conclusion PN can induce apoptosis of the human colon cancer cells. And the process may be carried out through the way of "NF-Κb→COX-2→PGE2".
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关 键 词: | 小白菊内酯 结肠肿瘤 细胞凋亡 |
Influence of parthenolide on the apoptosis of human colon cancer cells in vitro and its mechanism |
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Abstract: | |
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Keywords: | ParthenolideColon neoplasmsApoptosis |
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