二种阳极氧化电解液对钛表面细胞相容性的影响

目的:研究纯钛表面阳极氧化过程中电解液成份对其细胞相容性的影响。方法:在电流密度为70A/m2及200伏电压等条件下分别在0.03M甘油磷酸钙(Ca-GP)与0.15M醋酸钙混合液(混合组)及0.2M磷酸溶液中对纯钛样本表面进行阳极氧化处理。在阳极氧化及纯钛样本表面进行人类成骨细胞培养,以观察细胞毒性、细胞增殖及分化等细胞行为的改变。结果:细胞毒性试验结果表明,本研究中所用的电解液不会对阳极氧化后纯钛样本产生细胞毒性。所有样本表面经过1d、2d或4d细胞增殖培养后磷酸组与纯钛组之间没有显著性差异,而在0.03M甘油磷酸钙(Ca-GP)与0.15M醋酸钙混合液中进行阳极氧化处理后的纯钛样本,经过2d及4d成骨细胞培养后细胞增殖数量较纯钛组及磷酸组显著增高。细胞分化试验结果表明,除在4d时混合液组碱性磷酸酶活性较纯钛组及磷酸组显著性降低外,其余各组间无显著性差异。结论:对纯钛表面的阳极氧化处理过程中,电解液成份可对阳极氧化后纯钛表面的细胞相容性产生影响。

Effects of two kinds of electrolytes during anodization on biocompatibility of osteoblasts to pure titanium surface

Objective: Effects of anodizing electrolyte during anodization on biocompatibility of osteoblasts to pure titanium surface were investigated. Methods: The samples were anodized in the 0.2 M H3PO4 solution and the mixture of 0.03M calcium glycerophosphate (Ca-GP) and 0.15M calcium acetate (CA) with current density 70 A/m2 and voltage 200V. Osteoblasts (SaOS-2) were cultured on cp Ti and anodized titanium surfaces. Cytotoxicity, cell proliferation and cell differentiation were investigated. Results: Cytotoxicity test indicated that pure titanium specimens anodized in electrolytes used in this study had no cytotoxicity on human osteoblasts. There was no significant difference of cell proliferation between the specimens anodized in 0.2M H3PO4 and cp Ti after 1, 2 or 4 days cell culture. However, cell proliferation on the specimens anodized in the mixture of 0.03M calcium glycerophosphate (Ca-GP) and 0.15M calcium acetate (CA) was significantly enhanced than on cp Ti. The result of cell differentiation test indicated that the alkaline phosphatase (ALP) activity of mixture group was significantly reduced than the cp Ti group and H3PO4 group after 4 days cell culture. Conclusion: The cytocompatility of anodized pure titanium may be affected by the components of electrolyte.