AQP4参与雌激素对单胺类神经递质的调节作用

目的：探讨AQP4对雌激素调节神经递质作用的影响。方法：应用雄性AQP4基因敲除型CD1小鼠与野生型CD1小鼠，给予不同剂量雌激素后测定不同脑区中单胺类神经递质的含量。结果：AQP4基因敲除型雄性小鼠纹状体多巴胺（DA）及5-羟色胺（5-HT）、皮层去甲肾上腺素（NE）含量增加；海马NE、下丘脑DA水平下降。同时，给予雌激素后，野生型雄性CD1小鼠纹状体DA、5-HT含量及海马5-HT含量升高，皮层DA、5-HT水平及下丘脑NE、DA水平下降；而雌激素对AQP4敲除型雄性CD1小鼠单胺类递质无显著影响。结论：AQP4敲除可改变雌激素对单胺类递质水平的调节作用，对纹状体内DA水平的影响尤为显著。AQP4参与了雌激素对单胺类神经递质的调节。

AQP4 deficiency alters regulative effects of estrogen on monoamine neurotransmitters

AIM: To investigate whether aquaporin-4(AQP4)participates in the regulative action of estrogen on neurotransmitters (NT). METHODS: The monoamine neurotransmitters were determined by high performance liquid chromatography (HPLC) in different brain regions of male AQP4-knockout CD1 mice and wild-type CD1 mice after they were intraperitoneally injected with different doses of estradiol. RESULTS: The contents of DA and 5-HT in the striatum and NE in the cortex of AQP4-knockout CD1 were significantly increased, and the concentrations of NE in the hippocampus and DA in the hypothalamus were decreased. Treatment with estradiol increased the contents of DA and 5-HT in the striatum and that of 5-HT in the hippocampus, decreased the levels of DA and 5-HT in the cerebrum, and also reduced the concentrations of DA and NE in the hypothalamus in wild-type mice. However, these changes were not found in AQP4-knockout mice. CONCLUSION: The deficiency of AQP4 in mice alters the regulative effects of estrogen on monoamine neurotransmitters, indicating that AQP4 participates in regulating metabolism of neurotransmitters.