急性脑缺血大鼠模型血瘀证舌象表现的评价方法及其与血液流变学的关系

目的：用舌体组织血氧饱和度评价急性脑缺血大鼠血瘀证舌象表现，并探讨其与血流变异常的相关性，进一步验证急性脑缺血大鼠模型的血瘀证特征。方法：28只SD大鼠随机分为假手术对照组和模型组，用线栓法制备大鼠大脑中动脉阻塞缺血再灌注模型。缺血2h再灌注24h后观察大鼠舌体组织血氧饱和度、神经功能缺损以及血液流变学等指标。结果：与假手术对照组比较，脑缺血模型组大鼠舌体组织血氧饱和度和红细胞变形指数显著降低；全血和血浆黏度以及纤维蛋白原含量升高；凝血酶时间缩短。红细胞变形指数与舌体组织血氧饱和度呈正相关。结论：局灶性脑缺血大鼠模型急性期存在血瘀表现；舌组织血氧饱和度可以作为评价血瘀证动物模型舌象的客观指标。

Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia

OBJECTIVE: To study the tongue tissue blood oxygen saturation measurement for evaluating tongue manifestation of blood stasis syndrome, and to explore its correlation with blood rheological disorder in a rat model of acute transient brain ischemia. METHODS: Twenty-eight SD rats were randomly divided into sham-operated group and ischemia group. Middle cerebral artery occlusion was induced by thread in rats of the ischemia group. Tongue tissue blood oxygen saturation, neurological severity score and the changes of blood viscosity, red blood cell deformity, thrombin time and fibrinogen in the rats were measured after 24-hour reperfusion. RESULTS: Blood viscosity and the content of fibrinogen in the ischemia group were significantly higher than those in the sham-operated group. Red blood cell deformity, thrombin time and tongue tissue blood oxygen saturation in the ischemia group were decreased as compared with the sham-operated group. There was a positive correlation between red blood cell deformity and tongue tissue blood oxygen saturation. CONCLUSION: Tongue tissue blood oxygen saturation is a good measurement for evaluating blood stasis in a rat model of focal cerebral ischemia, and this model can be used as a rat model of stroke with blood stasis syndrome.