剪切力对血管内皮细胞表达IL-8的影响

本文利用改进的流室装置, 通过精密蠕动泵提供剪切力, 选择5dyne/cm2、10dyne/cm2、15dyne/cm2三种剪切力, 施加于体外培养的血管内皮细胞, 作用不同时间, 用RIA的方法检测流体动力学对血管内皮细胞表达IL-8的影响.结果表示, 不同剪切力作用内皮细胞后, IL-8的表达量与剪切力大小及作用时间有关, 为时间依赖性增长, 在5dyne/cm2组和10dyne/cm2组中, IL-8表达量明显高于空白对照组, 而15dyne/cm2组与对照组相比有下降趋势, 提示高剪切力有可能抑制IL-8的分泌.通过观察不同大小剪切力对血管内皮细胞表达IL-8的影响, 为探讨剪切力对动脉粥样硬化的影响提供一些数据.

Effects of Fluid Shear Stress on Interleukin-8 Experssion in Vascular Endothelial Cells

The objective was to study the effects of different shear stresses on expression of Interleukin-8(IL-8) in vascular endothelial cells and the mechanism of the contribution of hemodynamic forces to atherosclerosis. We utilized parallel plate flow chamber and pump to control the value(5dyne/cm 2,10dyne/cm 2 and 15dyne/cm 2) and duration (30min, 60min, 120min, 300min, and 420min)of shear stress in vascular endothelial cells.Expression of IL-8 was measured by RIA.The results showed that the first, the concentrations of IL-8 were time-dependently increased when different shear stresses were loaded on the endothelial cells, the second, the levels of IL-8 in the groups of 5dyne/cm 2 and 10dyne/cm 2 were higher than those in the control group. however, the level of IL-8 in the group of 15dyne/cm 2 was decreased.The result show that high shear stress might inhibit the secretion of IL-8 in vascular endothelial cells, to a certain degree, high shear stress could prevent the happening of atherosclerosis.