Gastrin-Releasing Peptide, a Bombesin-like Neuropeptide, Promotes Cutaneous Wound Healing |
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| Authors: | Yuji Yamaguchi MD PhD Ko Hosokawa MD PhD Yuka Nakatani BS Shigetoshi Sano MD PhD Kunihiko Yoshikawa MD PhD and Satoshi Itami MD PhD |
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| Institution: | Department of Dermatology, Osaka University Graduate School of Medicine, Suita-shi, Osaka, Japan. |
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| Abstract: | BACKGROUND: Little is known about the effects of neuropeptides on wound healing. OBJECTIVE: To investigate the effect of gastrin-releasing peptide (GRP), one of the bombesin-like neuropeptides, on wound healing. METHODS: The effects of GRP on cultured keratinocyte proliferation and migration were measured by BrdU uptake and in vitro scratch assay, respectively. Various concentrations of GRP ointments (0, 10(-9), 10(-8), 10(-7), 10(-6) M) were topically applied to 1.0 mm wounds on porcine flanks. RESULTS: GRP stimulated keratinocyte growth and locomotion in a dose-dependent manner. Topical administration of GRP accelerated macroscopic epidermal regeneration in a dose-dependent manner, as measured by planimetry. Histologic studies also showed that GRP promoted reepithelialization, including epidermal thickness as well as superficial skin coverage. CONCLUSION: Topical use of GRP may clinically accelerate wound healing of burns, injuries, chronic ulcers, and skin graft donor sites through the enhancement of keratinocyte growth and spreading. |
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