| 酶联免疫吸附法检测雨生红球藻超临界提取物中微囊藻毒素含量 |
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| 引用本文： | 万庆家,王朝军,倪广慧,龙祥,饶高雄.酶联免疫吸附法检测雨生红球藻超临界提取物中微囊藻毒素含量[J].云南中医学院学报,2010,33(3):38-40. |
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| 作者姓名： | 万庆家 王朝军 倪广慧 龙祥 饶高雄 |
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| 作者单位： | 1. 云南绿A生物工程有限公司,云南昆明,650106
2. 云南中医学院,云南昆明,650500 |
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| 基金项目： | 科技部科技型中小企业技术创新基金无偿资助项目 |
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| 摘 要： | 建立酶联免疫吸附法检测雨生红球藻超临界提取物中微囊藻毒素含量方法，并进行了方法学验证，标准曲线为y=-2．5032x＋2．504，R2=0．9994，检测范围0．1ng／mL～2ng／mL，平均加样回收率为93．3％，结果表明该方法准确可靠。运用该方法对10批20个样品进行了微囊藻毒素含量检测，均未检出囊藻毒素，表明采用超临界方法提取的雨生红球藻产品未受到微囊藻毒素污染，可以安全食用。
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| 关 键 词： | 酶联免疫吸附法 雨生红球藻 超临界提取物 微囊藻毒素 检测方法 |
Study on ELISA for Detecting Microcystins in the Extraction from Haematococcus pluvialis by Supercritical Fluid |
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| WAN Qing-jia,WANG Chao-jun,NI Guang-hui,LONG Xiang,RAO Gao-xiong.Study on ELISA for Detecting Microcystins in the Extraction from Haematococcus pluvialis by Supercritical Fluid[J].Journal of Yunnan College of Traditional Chinese Medicine,2010,33(3):38-40. |
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| Authors: | WAN Qing-jia WANG Chao-jun NI Guang-hui LONG Xiang RAO Gao-xiong |
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| Institution: | WAN Qing-jia, WANG Chao-jun, NI Guang- hui, LONG Xiang, RAO Gao- xiong (1. Yunnan Green A Biological Project Co. , Ltd, Kunming, Yunnan 650106, China; 2. Yunnan University of TCM, Kunming Yunnan 650500, China) |
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| Abstract: | We established ELISA for detecting microcystins in the extraction from Haematococcus pluvia l/s by Supercritical fluid. Method validation Results standard curve ： y = - 2. 5032x ＋ 2. 5043, ＂R2＂ value was O. 9994, microcystin - LR content wad in range of 0. 1 ng/mL - 2ng/ml, and the average of recovery rates of ELISA was 93.3%. Conclusion ： ELISA is an internationally advanced method for determi - nation of mierocystin - LR with high sensitivity, less interference, simplicity, rapidity and safety. Microcystin - LR were not detected in 20 tap sample from 10 different batches by this method, so this extraction from Haematococcus pluvialis by Su＇ percritical fluid can eat safety without pollution. |
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| Keywords: | ELISA Haematococcus pluvialis the extraction of supercritical fluid microcystin deteetion |
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