| 利用亚硝酸诱变原生质体筛选L-亮氨酸高产菌株的研究 |
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| 引用本文： | 齐秀兰,张志军,唐国新,李福德.利用亚硝酸诱变原生质体筛选L-亮氨酸高产菌株的研究[J].沈阳药科大学学报,1993(4). |
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| 作者姓名： | 齐秀兰 张志军 唐国新 李福德 |
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| 作者单位： | 沈阳药学院微生物学教研室，沈阳药学院微生物学教研室，沈阳药学院微生物学教研室，沈阳药学院微生物学教研室 55期微生物制药专业学生，55期微生物制药专业学生 |
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| 摘 要： | 本实验以L-亮氨酸产生菌黄色短杆菌CFN-19为出发菌株。我们首先对CFN-19菌株制备原生质体,原生质体制备率为95.1%,再生率为25%,然后对原生质体进行亚硝酸、利福平、氯化锂复合诱变处理,从再生菌株中筛选出一株高产菌株H_(6-66),产酸量由原来的23.1mg/ml提高到32.6mg/ml,提高率达41%.同时又对H_(6-66)菌株进行遗传稳定性考察,考察结果H_(6-66)菌株是高产稳定菌株。
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| 关 键 词： | L-亮氨酸 原生质体 诱变 |
THE SELECTION OF HIGH YIELD L-LEUCINE STRAIN THROUGH MUTATION OF PROTOPLAST INDUCED BY NITRIC ACID |
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| Qi Xiulan Zhang Zhijun Tang Guoxin Li Fude.THE SELECTION OF HIGH YIELD L-LEUCINE STRAIN THROUGH MUTATION OF PROTOPLAST INDUCED BY NITRIC ACID[J].Journal of Shenyang Pharmaceutical University,1993(4). |
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| Authors: | Qi Xiulan Zhang Zhijun Tang Guoxin Li Fude |
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| Institution: | Qi Xiulan Zhang Zhijun Tang Guoxin Li Fude Department of Microbiology |
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| Abstract: | In this study, L-leucine producining strain Brevibac terium flavum CFN-19 was used as initial organism. First, the protoplast of CFE-19 was prepared,the preparation frequency of protoplast being 95.1% and the regeneration frequency of protoplast being 25% under optimum conditions. Then the protoplast was treated with nitric acid combined with Rifampin and Licl, high yield producer H_(6-68) was selected from regenerated strains and the production of L-leucine was increased by 41%, from 23.1mg/ml to 32.6mg/ ml. Investigation of its genetic stability confirms that H_(6-66) is a stable strain. |
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| Keywords: | L-leucine Protoplast Mutation |
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