| 农抗5102产生菌工程菌株WH─1的稳定性 |
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| 引用本文： | 赵国方,赵步贵,覃重军,梁蓉芳,周启. 农抗5102产生菌工程菌株WH─1的稳定性[J]. 中国抗生素杂志, 1996, 0(5) |
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| 作者姓名： | 赵国方 赵步贵 覃重军 梁蓉芳 周启 |
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| 作者单位： | 华中农业大学微生物系 |
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| 基金项目： | 湖北省自然科学基金 ,国家教委博士点专项基金 |
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| 摘 要： | 无论是在连续传代的过程中，还是在不同温度条件下保存的过程中，工程菌株ＷＨ－１均不稳定，其不稳定性主要表现在培养特征的变化和产生抗生素性能的衰退两个方面，且都是一个由量变逐渐转化为质变的过程。采用连续多次单孢子分离筛选的方法可以获得比较稳定的高产菌株。本实验主要通过５次单孢子分离筛选，得到了ＷＨ－１－１、ＷＨ－１－２、ＷＨ－１－３、ＷＨ－１－４和ＷＨ－１－５共５个较稳定的高产菌株
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| 关 键 词： | 农抗5102产生菌工程 菌株WH－1 稳定性 |
The stability of the genetic engineering strain WH 1 of agricultural antibiotic 5102 producing strain 10 22 |
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| Zhao Guofang,Zhao Bugui,Qin Zhongjun,Liang Rongfang and Zhou Qi. The stability of the genetic engineering strain WH 1 of agricultural antibiotic 5102 producing strain 10 22[J]. Chinese Journal of Antibiotics, 1996, 0(5) |
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| Authors: | Zhao Guofang Zhao Bugui Qin Zhongjun Liang Rongfang Zhou Qi |
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| Abstract: | The instability of the genetic engineering strain WH 1 of agricultural antibiotic 5102 producing strain 10 22 was not altered either by serial subculture or by modifying the preserved temperature conditions. It evidenced itself by the degenerating of the antibiotic productivity, as well as changing the cultural characteristics. The process progressed stepwisely. By means of multiple successive single spore selection approach, we could isolate 5 comparatively stable high yield strains: WH 1 1, WH 1 2, WH 1 3, WH 1 4, WH 1 5. |
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| Keywords: | Antibiotic 5102 producing strain Genetic engineering strain WH 1 Stability |
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