大黄素在小鼠皮肤移植中的免疫抑制作用的实验研究

目的：研究大黄素对小鼠皮肤移植的免疫抑制作用，探讨其可能的作用机制。方法：建立小鼠C57BL／6→BALB／c异体皮肤移植模型。以大黄素和环孢素A（CsA）分别灌胃，观察不同药物及药物浓度对小鼠皮肤移植物存活时间的影响。采用ELISA法检测大黄素和CsA对皮肤移植小鼠血浆IL-2表达水平的影响，组织学观察不同药物浓度对移植物病理变化的影响。结果：大黄素显著降低小鼠血浆内IL-2的浓度，同时伴有移植物的存活时间的延长，组织学观察发现大黄素可以减轻移植排斥反应的强度，其作用表现出量效依赖性，并与CsA具有协同性。结论：大黄素能够减轻C57BL／6→BALB／c异体皮肤移植的排斥反应强度，其作用机制可能与抑制IL-2的分泌有关。

Immunosuppressive effects of emodin on skin transplantation in mice

AIM： To investigate the immunosuppressive effects of emodin on skin transplantation in mice and its immunosuppressive mechanism. METHODS ： C57BL/6→BALB/c skin trans-plantation models were established and then treated with different concentrations of emodin and cyclosporine A （CsA）. The different mean survival times （MST） of different groups were observed. Changes of plasma cytokines （IL-2） in mouse skin transplantation models were determined by ELISA and histological changes at different concentrations of emodin and eyclosporine A （CsA） were investigated. RESULTS： In the group treated with emodin, the MST of skin graft was prolonged and production of plasma IL-2 decreased. The histological results suggested that emodin relieved the graft immunological damage in a dose-dependent manner and exerted synergistic effect with CsA. CONCLUSION： Emodin exerts some immunosuppressive effects on mouse skin transplantation possibly by suppressing IL-2.