Inhibition of human neutrophil migration by supernatants from Hodgkin''s disease-derived cell lines

The contribution of defective neutrophil function to the increased susceptibility to infection observed in patients with Hodgkin's disease is unclear. We describe cell-directed inhibition of normal human neutrophil migration by serum-free culture supernatants of the Hodgkin-derived cell line L428 KSA, tentatively termed Hodgkin-derived leucocyte factor (HDLF). This factor inhibits both random migration and migration toward different chemoattractants, appears to bind to the cell surface and is stable at 56 degrees C but destroyed at 100 degrees C. Hodgkin-derived leucocyte factor also stimulates basal neutrophil superoxide production but the cells remain fully responsive to n-formyl-methionylleucylphenylalanine. Gel filtration chromatography shows a single peak of migration-inhibitory and superoxide-stimulatory activity at approximately 70,000 g mol-1. Hodgkin-derived leucocyte factor migration inhibition persists in neutrophils from a patient with chronic granulomatous disease. Activity of HDLF is completely destroyed by trypsin but unaffected by the protease inhibitor phenyl-methylsulphonylfluoride. Hodgkin's factor appears to be different from previously described neutrophil migration inhibitory factors.