Lack of association between CTLA-4 and PDCD1 polymorphisms and acute rejection in German liver transplant recipients |
| |
Authors: | Hansjörg Thude Anna Dorothea Schipler Andras Treszl Sven Peine Martina Koch Martina Sterneck Björn Nashan |
| |
Institution: | 1. University Hospital Hamburg-Eppendorf, Department of Hepatobiliary and Transplant Surgery, Martinistraβe 52, 20246 Hamburg, Germany;2. University Hospital Hamburg-Eppendorf, Department of Medical Biometry and Epidemiology, Martinistraβe 52, 20246 Hamburg, Germany;3. University Hospital Hamburg-Eppendorf, Institute for Transfusion Medicine, Martinistraβe 52, 20246 Hamburg, Germany |
| |
Abstract: | Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell-death 1 (PDCD1) are two genes encoding coinhibitory immunoreceptors that are involved in regulation transplant rejection and tolerance induction. Thus, CTLA-4 and PDCD1 may be good candidate genes to evaluate in liver transplant rejection. In this retrospective study, we investigated whether four functional single nucleotide polymorphisms (SNP) of the CTLA-4 gene and PDCD1 gene were associated with susceptibility to liver transplant rejection. The SNPs −1772T > C (rs733618), −1661A > G (rs4553808) of the CTLA-4 gene, and the SNPs 7146G > A (rs11568821), 7209C > T (rs41386349) of the PDCD1 gene were genotyped by polymerase chain reaction allele specific restriction enzyme analysis (PCR-ASRA) in 100 liver recipients with acute rejection, 104 liver transplant recipients without acute rejection and 100 healthy control individuals. For the selected SNPs we did not detect any significant difference in genotypic and allelic frequencies between liver transplant recipients with and without acute rejection. In conclusion, our results suggest that the tested SNPs may not be associated with susceptibility to acute liver transplant rejection in a Caucasian population. |
| |
Keywords: | AZA azathioprine bp base pairs CD cluster of differentiation Cl confidence interval CNI calcineurin inhibitor CTLA-4 cytotoxic T-lymphocyte-associated antigen 4 sCTLA-4 soluble cytotoxic T-lymphocyte-associated antigen 4 f female HCV hepatitis C virus HBV hepatitis B virus LTX liver transplantation m male MMF mycophenolate mofetil mTOR mammalian of rapamycin OR odds ratio PCR polymerase chain reaction PCR-ASRA polymerase chain reaction-allele specific restriction enzyme analysis PDCD1 programmed cell-death 1 SD standard deviation SNP single nucleotide polymorphism |
本文献已被 ScienceDirect 等数据库收录! |
|