Enterococcus faecium resistente a glucopéptidos. Análisis del genotipo de resistencia,virulencia y líneas genéticas |
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Authors: | María López Míriam J Álvarez-Martínez Francesc Marco Carmen Torres |
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Institution: | 1. Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, España;2. Servicio de Microbiología, CDB (Centro de Diagnóstico Biomédico), Hospital Clínic, Facultad de Medicina, Barcelona, España;3. Centre for International Health Research (CRESIB), Hospital Clínic-Universidad de Barcelona, Barcelona, España |
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Abstract: | ObjectiveThe objective of this study was to analyse the genotypic characteristics of all Enterococcus isolates with acquired vancomycin resistance (VRE) recovered in the Hospital Clinic (Barcelona, Spain) in a period of three years and two months.MethodsAll VRE isolated in the referred Hospital in the period January 2004-March 2007 were included in the study. The vancomycin resistance mechanism was investigated, as well as other antibiotic resistance mechanisms. Isolates were also typed by pulsed-field-gel-electrophoresis (PFGE) and multi-locus-sequence-typing (MLST).ResultsThirty-nine VRE were recovered, all being identified as E. faecium, representing 2% of total enterococci obtained in that period. Thirty-eight of them carried the vanA gene, and one isolate the vanB2 gene. The 39 VRE were classified into 13 different pulsotypes (A-M), with one main pulsotype, A, which included 13 isolates. The sequence type was identified by MLST in 24 VRE (with unrelated or closely-related PFGE patterns), and they were ascribed to the clonal complex CC17, but two classified as CC9. All VRE showed a multiresistance phenotype, including, in most cases ampicillin, ciprofloxacin, erythromycin, streptomycin, gentamicin, kanamycin and chloramphenicol, harbouring multiple antibiotic resistance genes. The presence of esp and/or hyl genes was identified in 37 VRE.ConclusionAll VRE, but one, showed the vanA genotype and they were mostly ascribed to the high-risk clonal complex CC17. |
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Keywords: | Enterococcus faecium resistente a vancomicina Multi-locus-sequence-typing vanA vanB2 |
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