Haplotypes of the IL-1 gene cluster are associated with gastroesophageal reflux disease and Barrett’s esophagus |
| |
Authors: | Lydie Izakovicova Holla Petra Borilova Linhartova Barbara Hrdlickova Filip Marek Jiri Dolina Vladimir Rihak Zdenek Kala |
| |
Affiliation: | 1. Department of Pathophysiology, Medical Faculty, Masaryk University Brno, Czech Republic;2. Department of Surgery, University Hospital Brno-Bohunice, Czech Republic;3. Department of Gastroenterology, University Hospital Brno-Bohunice, Czech Republic;4. Regional Thomas Bata Hospital, Zlín, Czech Republic |
| |
Abstract: | ObjectivesGastroesophageal reflux (GERD) is a one of the major public health problem that can lead to reflux esophagitis (RE), Barrett’s esophagus (BE), and esophageal adenocarcinoma (EAC). The aim of our study was to determine the impact of IL-1 gene polymorphisms on the development of GERD, RE and BE.MethodsThree hundred and thirty-three Czech patients with gastroesophageal reflux and 165 healthy controls were included in this case-control study. Four polymorphisms in the genes of the IL-1 cluster [IL-1A(-889C/T), IL-1B(−511C/T), IL-1B(+3953C/T), and IL-1RN(VNTR)] were analyzed.ResultsSignificant differences were found in IL-1RN 1/2 genotype between patients with GERD/RE and controls and in IL-1B+3953 T allele between patients with BE and healthy subjects. In addition, complex analysis revealed differences in IL-1 haplotype frequencies between the groups. Specifically, the haplotype TCCL was significantly more frequent (p = 0.016) in GERD patients than in controls and the haplotype CCCL more frequent (p = 0.008) in RE patients than in controls. However, in patients with BE, frequency of haplotype TCTL was lower (p = 0.05) and haplotypes CTCL and TCCL were higher (p = 0.03 and p = 0.02) in comparison with the controls.ConclusionsOur results suggest that IL-1 haplotypes may be associated with susceptibility to GERD, RE and BE. |
| |
Keywords: | BE, Barrett&rsquo s esophagus CI, confidence intervals DNA, deoxyribonucleic acid DU, duodenal ulcer EAC, adenocarcinoma of the esophagus EE, erosive esophagitis EC, esophageal cancer GAC, gastric adenocarcinoma GERD, gastroesophageal reflux disease GIQLI, gastrointestinal quality of life index GU, gastric ulcer HWE, Hardy&ndash Weinberg equilibrium IL-1, interleukin-1 LD, linkage disequilibrium LES, lower esophageal sphincter NERD, non-erosive reflux disease OR, odds ratio PCR, polymerase chain reaction RE, reflux esophagitis RFLP, restriction fragment length polymorphism SNP, single nucleotide polymorphism VNTR, variable number tandem repeat |
本文献已被 ScienceDirect 等数据库收录! |
|