Función efectora de linfocitos T CLA+ sobre queratinocitos autólogos en psoriasis

BackgroundCutaneous lymphocyte antigen (CLA) is expressed by a subgroup of memory T cells that exhibit skin homing and are implicated in cutaneous T-cell-mediated diseases.Material and methodsExpression of genes associated with psoriasis was analyzed in keratinocytes taken from patients and healthy individuals and cultured under different conditions, including activation using supernatants from CLA⁺ T lymphocytes activated with anti-CD3 and anti-CD28 antibodies.ResultsKeratinocytes from psoriasis patients activated by CLA⁺ T lymphocytes expressed higher levels of interferon-inducible protein 10, HLA-DR, intercellular cell adhesion molecule 1, and inducible nitric oxide synthase.ConclusionsOur results suggest that we have developed an in vitro model that will allow analysis of the effector role of CLA⁺ T lymphocytes on keratinocytes in psoriasis. This model may allow the identification of genes involved in the pathology of psoriasis through induction by CLA⁺ T lymphocytes.