局灶性脑缺血再灌注大鼠Nestin的表达

目的了解局灶性脑缺血再灌注大鼠脑组织中Nestin的表达特点。方法采用改良的线栓法制备大脑中动脉阻塞(MCAO)2h后不同再灌注时间段(6h、12h、24h、72h、7d、14d)的大鼠局灶性脑缺血模型,应用免疫组织化学技术检测Nestin的表达。结果脑缺血再灌注后6h,Nestin 表达即有增加,至3d时Nestin 表达显著增强,Brdu /Nestin 细胞也已明显可见,至7d时Nestin 表达、Brdu /Nestin 细胞数目均达顶峰,至再灌注14dNestin 表达及Brdu /Nestin 细胞数目均已明显回落。结论脑缺血可引发大鼠Nestin的表达上调,并可诱导神经干细胞增殖。

Expression of Nestin Following Focal Cerebral Ischemia-Reperfusion in Rats

Objective To study the expession of Nestin after focal ischemia-reperfusion in rats. Methods The model of acute reperfusion injury after cerebral ischemia in rat was made by middle cerebral artery occlusion. All animals were allowed 2 h ischemia and sacrificed after different durations of reperfusion (6 h, 12 h, 24 h, 72 h,7 d,14 d). The expression of Nestin was detected with an immunohistochemical approach. Results The number of Nestin-immunoreactive cells at 6 h after reperfusion in the ischemic side started to increase markedly and showed significant difference, compared with the Sham group (P<0.05). The expression of Nestin and the number of Nestin positive cells with Brdu labeled peaked at 7 d after reperfusion and then were down-regulated at 14 d. Conclusion The study implies cerebral ischemia could upregulate the expression of Nestin and induce the proliferation of nervous stem cells.