差异显示基因EEG1在原发性肝细胞癌中的表达及其临床意义

目的研究和克隆原发性肝细胞癌（HCC）相关基因，探索其在肝癌发病中的临床意义。方法采用mRNA差异显示技术，分析肝细胞癌和癌旁正常肝组织间基因的差异表达，获得差异表达基因片段，对其进行克隆，测序，并用RTPCR方法，检测所获得的基因在44对肝细胞癌及相应癌旁正常肝组织中差异表达的情况，分析该基因表达与HCC临床病理特征的关系。结果克隆了一个肝癌相关基因EEG1，其在肝癌组织中的表达水平明显低于癌旁正常肝组织，仅为正常组织表达量的0．586±0．346倍（P〈0．05）。EEG1基因在肝癌组织中的表达下调与肝癌的早期分化程度密切相关（P〈0．05）。结论差异表达基因EEG1可能在HCC的早期发病中起着重要作用，有望成为肝癌的早期诊断指标。

EEG1 Differential expression in primary hepatocellular carcinoma and its clinical significance

Objective To clone and study primary hepatocellular carcinoma(HCC)-related gene,and explore its clinical significance in the pathogenesis of HCC.Methods mRNA differential display technology was applied to detecting the differential expression in HCC tissue and cancer adjacent normal liver tissue.The differential expressed gene fragment was acquired.Its PCR clones were scquenced and checked for significant alignments in GenBank.The distribution in normal liver tissue and HCC tissue was examined by RT-PCR.The correlation was analyzed between the differential expressed gene and clinicopathologic characleristics of HCC.Results A HCC-related gene EEG1 was cloned.As compared with that in normal liver tissue,EEG1's expression level in HCC tissue was significantly lower(only 0.586 ± 0.346 times,P＜0.05).Downregulation of EEG1 in HCC tissue was closely associated with early differentiation of HCC(P＜0.05).Conclusion The differential expressed gene EEG1 may play an important role in onset of HCC,which may be used as a potential early tumor gene marker for the diagnosis of HCC.