特发性肺纤维化患者支气管/肺泡上皮细胞凋亡和Fas/FasL基因表达

目的：检测细胞凋亡、Fas/FasL mRNA及其蛋白在特发性肺纤维化患者肺泡/支气管上皮细胞的表达,探讨细胞凋亡和促凋亡信号在此疾病中的意义。方法：应用末端原位杂交（TUNEL）、原位杂交、免疫组化技术,对12例特发性肺纤维化患者的肺活检组织（IPF组）及10例正常肺组织（对照组）检测了细胞凋亡、Fas/FasL mRNA及其蛋白表达的变化。结果：特发性肺纤维化组肺泡/支气管上皮细胞凋亡指数上调,明显高于对照组（P〈0.01）;IPF组肺泡/支气管上皮细胞中Fas/FasL mRNA及其蛋白表达上调,高于对照组（P〈0.01）;IPF组肺泡/支气管上皮细胞凋亡指数与其Fas/FasL mRNA及蛋白表达之间均无明显相关（P〉0.05）。结论：肺纤维化时肺泡/支气管上皮细胞凋亡上调,Fas/FasL mRNA及其蛋白表达增强,在肺纤维化发生、发展中起重要作用。

Expression of cell apoptosis and the change of Fas/FasL gene in epithelial cells in idiopathic pulmonary fibrosis

Objective To investigate the changes and significance of cell apoptosis,Fas/FasL mRNA and protein in epithelial cells in idiopathic pulmonary fibrosis.Methods TUNEL,immunohistochemistry and in situ hybridization were used to detect the change of cell apoptosis,Fas/FasL mRNA and protein in 12 lung tissues from patients with idiopathic pulmonary fibrosis(IPF group) and 10 normal lung parenchyma specimens(control group).Results Compared with control group,the apoptosis index in alveolar epithelial cells and bronchial cells of IPF group was higher(P<0.01).The expression of Fas,FasL mRNA and protein in the pulmonary fibrosis group was higher than that of control group(P<0.01).There was no statistical correlation of the apoptosis index,Fas/FasL mRNA and protein expression(P>0.05).Conclusion The apoptosis index of epithelial cells,Fas/FasL mRNA and protein are up-regulated in epithelial cells in lung tissue of pulmonary fibrosis,which may play an important role in the development of the disease.