l-DOPA treatment reverses the motor alterations induced by manganese exposure as a Parkinson disease experimental model |
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Authors: | Jose Luis Ordoñez-Librado Veronica Anaya-Martinez Ana Luisa Gutierrez-Valdez Enrique Montiel-Flores David Reyes Corona Daniel Martinez-Fong Maria Rosa Avila-Costa |
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Institution: | 1. Laboratorio de Neuromorfologia, FES Iztacala, UNAM, Av. de los Barrios 1, Tlalnepantla 54090, Mexico;2. Departamento de Fisiología, Biofisica y Neurociencias, CINVESTAV-IPN, Mexico Av. IPN 2508, CP 07360, Mexico |
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Abstract: | This investigation was designed to determine whether l-DOPA treatment improves the motor alterations observed after divalent and trivalent manganese (Mn) mixture inhalation on mice to ensure that the alterations are of dopaminergic origin. CD-1 male mice inhaled a mixture of 0.04 M manganese chloride (MnCl2) and manganese acetate (Mn(OAc)3), 1 h twice a week for 5 months. Before Mn exposure, animals were trained to perform motor function tests and were evaluated each week after the exposure. Overall behavior was assessed by ratings and by videotaped analyses; by the end of Mn exposure period, 10 mice were orally treated with 7.5 mg/kg l-DOPA. After 5 months of Mn-mixture inhalation striatal dopamine content decreased 71%, mice developed evident deficits in motor performance manifested as akinesia, postural instability and action tremor; these alterations were reverted with l-DOPA treatment. Our results suggest that the motor alterations induced by the inhalation of the combination of MnCl2/Mn(OAc)3 are related to nigrostriatal dopaminergic function providing new light on the understanding of manganese neurotoxicity as a suitable Parkinson disease experimental model. |
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Keywords: | Parkinson disease experimental model Manganese inhalation Motor behavior l-DOPA Dopamine |
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