| 四氯化碳中毒大鼠肝脏鞘糖脂的研究 |
| |
| 引用本文： | 汤乃梅,张新波.四氯化碳中毒大鼠肝脏鞘糖脂的研究[J].中华劳动卫生职业病杂志,1994,12(5):269-271. |
| |
| 作者姓名： | 汤乃梅 张新波 |
| |
| 作者单位： | 北京医科大学神经科学研究中心，北京医科大学生理教研室，大连医学院生化教研室 |
| |
| 摘 要： | 观察了四氯化碳（ＣＣｌ４）中毒时大鼠肝脏鞘糖脂总量及其组成的变化。结果表明：ＣＣＩ４染毒后，神经节苷脂和中性鞘糖脂总量均有所增加；神经节苷脂ＧＤ３和ＧＭ１组分分别在染毒１２ｈ和２４ｈ后明显增加；染毒２４ｈ后还出现了正常鼠肝中未检测出的ＧＭ２组分。中性鞘糖脂在染毒２４ｈ和４８ｈＣＴＨ组分明显增加，并于４８ｈ出现了ＣＸＨ未知新组分。这提示ＣＣＩ４中毒后，鼠肝的鞘糖脂组成与含量均有变化，但其意义尚待阐明。
|
| 关 键 词： | 四氯化碳中毒，鞘糖脂，肝脏，层析 |
STUDIES ON GLYCOSPHINGOLIPID CHANGES IN CARBON TETRACHLORIDE INTOXICATED
RAT LIVER |
| |
| Tang Naimei,Zhang Xinbo,Xia Quan,et al Research Center of
Neurosciences,Beijing Medical University l.STUDIES ON GLYCOSPHINGOLIPID CHANGES IN CARBON TETRACHLORIDE INTOXICATED
RAT LIVER[J].Chinese Journal of Industrial Hygiene and Occupational Diseases,1994,12(5):269-271. |
| |
| Authors: | Tang Naimei Zhang Xinbo Xia Quan Research Center of
Neurosciences Beijing Medical University l |
| |
| Institution: | Tang Naimei,Zhang Xinbo,Xia Quan,et al Research Center of
Neurosciences,Beijing Medical University l00083 |
| |
| Abstract: | Rats were treated by gastrogavage
of CCI4 and sacrified after l2,24,48 hours,respectively. Using solvent ex-traction and column
chromatography,the total gangliosides( GLS))and totaI neutral glycosphingolipid(NGSL) were
separated and purified,and the amount were expressed by LBSA and sphingosine
contents,respectively.TheresuIts showed that CCI4 could induce the increase of the content of
glycosphingolipids(including GLs and NGSL)inrat liver.After 12 to 24 h of intoxication,GD3
significantly increased, and after 24 to 48 h of intoxication, GM1 and CTH increased, and GM2
and CXH( an unidentified NGSL component) which were not seen in normal rat liver,appeared in
the intoxicated liver.These results suggested that CCI4 intoxication might cause the increase of
totalGSL and also the increase of GM2,GD,GM1,CTH,CXH. |
| |
| Keywords: | Carbon tetrachloride intoxication Glycosphingolipid Liver Chromatography |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
