Urine proteomes of healthy aging humans reveal extracellular matrix (ECM) alterations and immune system dysfunction |
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Authors: | M Bakun G Senatorski T Rubel A Lukasik P Zielenkiewicz M Dadlez L Paczek |
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Institution: | 1. Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawinskiego 5a, 02-106, Warsaw, Poland 2. Department of Immunology, Transplantology and Internal Diseases, Transplantation Institute, Medical University of Warsaw, ul. Nowogrodzka 59, 02-006, Warsaw, Poland 3. Institute of Radioelectronics, Warsaw University of Technology, ul. Nowowiejska 15/19, 00-665, Warsaw, Poland 4. Bioinformatics Department, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawinskiego 5a, 02-106, Warsaw, Poland 5. Department of Biology, Warsaw University, ul. Miecznikowa 1, 02-096, Warsaw, Poland
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Abstract: | Aging is a complex physiological process that poses considerable conundrums to rapidly aging societies. For example, the risk of dying from cardiovascular diseases and/or cancer steadily declines for people after their 60s, and other causes of death predominate for seniors older than 80 years of age. Thus, physiological aging presents numerous unanswered questions, particularly with regard to changing metabolic patterns. Urine proteomics analysis is becoming a non-invasive and reproducible diagnostic method. We investigated the urine proteomes in healthy elderly people to determine which metabolic processes were weakened or strengthened in aging humans. Urine samples from 37 healthy volunteers aged 19–90 years (19 men, 18 women) were analyzed for protein expression by liquid chromatography–tandem mass spectrometry. This generated a list of 19 proteins that were differentially expressed in different age groups (young, intermediate, and old age). In particular, the oldest group showed protein changes reflective of altered extracellular matrix turnover and declining immune function, in which changes corresponded to reported changes in cardiovascular tissue remodeling and immune disorders in the elderly. Thus, urinary proteome changes in the elderly appear to reflect the physiological processes of aging and are particularly clearly represented in the circulatory and immune systems. Detailed identification of “protein trails” creates a more global picture of metabolic changes that occur in the elderly. |
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Keywords: | Aging Extracellular matrix alterations Mass spectrometry Immune system dysfunction Proteomics Urine proteome |
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