盐酸纳洛酮治疗大鼠急性颅脑损伤的药效学观察

目的观察盐酸纳洛酮(金尔伦)在大鼠急性颅脑损伤实验模型中促进神经功能恢复的治疗作用,并做量效分析。方法SD大鼠250只,采用Feenly自由落体撞击法建立颅脑损伤模型,随机分成六组,于损伤后30min开始给药。前四组每天分别给予金尔伦0.3mg/kg、1mg/kg、3mg/kg和9mg/kg腹腔注射;阳性对照组:给予胞磷胆碱钠2mg/只腹腔注射;阴性对照组:给予0.5ml/只生理盐水腹腔注射。每天进行MNSS神经功能评分,最长疗程为14d。伤后2d和4d每组随机取8只大鼠,通过干—湿重法计算脑组织的含水量。结果金尔伦治疗组大鼠的神经功能恢复情况明显优于其他两组(P<0.01)。金尔伦1、3、9mg/kg三组的情况优于0.3mg/kg组(P<0.05),而这三组之间没有显著性差异(P>0.05)。金尔伦治疗组大鼠的脑含水量明显低于对照组(P<0.05);金尔伦内部各实验组之间,0.3mg/kg组的脑含水量高于其他三组(P<0.05),其他三组之间无显著性差异(P>0.05)。结论金尔伦能够降低大鼠急性颅脑损伤后的脑水肿,对大鼠的神经功能恢复有明显的促进作用,并在一定范围内随着剂量的增加效果更显著。

The pharmaceutic effect of Nalxotone on SD rats with head trauma

Objective To investigate the pharmaceutic effect of Nalxotone on nerve functions of SD rats with brain injury. Methods 250 SD rats with head trauma by the free-falling-body-crash were divided into six groups randomly. The rats of 1-4 trial groups were injected with Nalxotone transpeniponeally by 03mg, 1mg, 3mg and 9mg per kilogram half an hour after injury, meanwhile the control-groups were given 2mg Citicoline Sodium for injection and 0.5ml normal saline per rat respectively. Score of MNSS on every rat was recorded every day, the longest time was 14 days. The brain edemas of 8 rats of each group were measured with wet-dry weight method at the second and the fourth day after head trauma. Results The Nerve function of rats in Nalxotone group was better than the two control groups (P<0.01), and that of 1?3?9 mg/kg Nalxotone group were better than 0.3mg/kg (P<0.05),but there were no significant difference among these three Nalxotone groups (P >0.05). The brain edemas of rats in Nalxotone group were lighter than the control groups, and that of 1?3?9 mg/kg group were lighter than 0.3mg/kg (P<0.05),but there were no significant difference among these three groups(P >0.05). Conclusions Nalxotone is able to decrease the brain edema of rat with brain injury, promote the nerve function recovery, and the treatment effect changes with the dosage during some range.