呼和浩特市鼠类携带汉坦病毒的基因分型

目的对呼和浩特市流行的汉坦病毒进行基因分型,为制定肾综合征出血热防制对策提供科学依据。方法对经间接免疫荧光试验初筛为阳性的鼠肺标本用汉坦病毒型特异性引物,以巢式逆转录-聚合酶链反应(RT-PCR)扩增部分M和S基因片段上的目的核苷酸序列,纯化回收测序后,将扩增片段的核苷酸序列与已知病毒的核苷酸序列进行比较,以明确汉坦病毒的基因型及基因亚型。结果呼和浩特市33份阳性鼠肺中,其中32份为褐家鼠肺,1份为小家鼠肺,经巢式RT-PCR扩增,用汉城病毒型特异性引物共扩增出部分S、M片段(G1、G2区)69个,经同源性和系统发生分析,褐家鼠和小家鼠所携带汉坦病毒均为汉城型S1亚型。结论呼和浩特市家鼠中流行的汉坦病毒为汉城型,与流行病学分型一致。

Genotype of hantavirus carried by the rodents in Hohhot city

Objective To identify the genotype of hantavirus(HV) carried by the rodents in Hohhot. Methods The partial S and M segment were amplified from the HV antigen positive rodents lungs detected by indirect immunofluorescence assay with nested RT-PCR with genotype-specific primers, and then were sequenced and compared with other known hantaviruses. Results 69 partial S and M segment were amplified from 33 HV antigen-positive rodents lungs, 32 of which from Rattus norvegicus and 1 from Mus musculus. The genotype of hantavirus carried by them is seoul virus(SEOV), and the sub-genotype is SEOV-1 by homology and phylogenesis analysis. Conclusion The genotype of hantavirus carried by rat in Hohhot is SEOV and is consistent with epidemiology typing.