Population genetic data for 12 X-STR loci in the Northern Han Chinese and StatsX package as tools for population statistics on X-STR |
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| Institution: | 1. Department of Family Genetics, Norwegian Institute of Public Health, P.O. Box 4040 Nydalen, NO-0403 Oslo, Norway;2. Department for Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway;3. Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, SE-587 58 Linköping, Sweden;4. Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden;1. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany;2. Human Genetics Laboratory, University of Madeira, Funchal, Portugal;3. DNA Diagnostic Laboratory (LDD), Institute of Biology, State University of Rio de Janeiro (UERJ), Brazil;4. IPATIMUP, Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal;5. Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal;2. National Forensic Laboratory, Police, Ministry of the Interior, Vodovodna 95, 1000 Ljubljana, Slovenia;3. The State Forensic Medicine Service under the Ministry of Justice of the Republic of Lithuania, Vilnius, Lithuania;4. Scientific Support Section, Subdivision of Biological and Biochemical Examinations and Analyses F.S.D.—Hellenic Police, Athens, Greece;1. Department of Family Genetics, Norwegian Institute of Public Health, P.O. Box 4040, Nydalen, NO-0403 Oslo, Norway;2. Department for Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway |
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| Abstract: | The X-chromosomal short tandem repeat (X-STR) has the advantage in female traces identification against male contamination and in complex kinship cases. In this study, a total of 516 Northern Han Chinese were genotyped using the Investigator® Argus X-12 QS Kit and pairwise genetic distances (FST) were measured between Northern Han Chinese and 35 published populations using the same 12 X-STR loci in four presumed linkage groups (LG). Meanwhile, the StatsX package was compiled to aid the analysis of population statistics on X-STR. Off-ladder alleles were investigated by Sanger sequencing or next generation sequencing (NGS). The results showed that high combined PDM, PDF, MECKrüger, MECKishida, MECDesmarais and MECDesmarais Duo based on allele frequencies were achieved as 0.999999998, >0.999999999, 0.999996425, 0.999999993, 0.999999993 and 0.999998732, respectively, so did they based on haplotype frequencies. Averaged FST and multidimensional scaling (MDS) plot generally mirrored with the biogeography distribution of the studied populations and their historical relationships. A total of 16 unique off-ladder alleles were observed in this experiment, four of which have not been reported yet. The StatsX package could obtain full concordance with established software. Overall, the Investigator® Argus X-12 QS Kit may provide high polymorphic information for forensic identification and kinship analysis in the Northern Han Chinese population, and the StatsX package can make the workflow smoother for researchers to do population statistical analysis on X-STR. |
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| Keywords: | Population genetics X-chromosomal short tandem repeat (X-STR) Northern Han Chinese StatsX package |
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