平胃散对湿困脾胃证肠黏膜及离子泵损伤修复作用研究

目的:探讨湿困脾胃证模型肠黏膜屏障功能障碍及平胃散的修复作用。方法:40只大鼠随机分为空白对照组、模型组、治疗组、自然恢复组,每组10只.建立湿困脾胃证大鼠模型,观测各组大鼠血清D-乳酸含量、血清二胺氧化酶(DAO)活性、空肠组织匀浆谷氨酰胺(Gln)浓度和Na+-K+-ATPase,Ca2+-ATPase活力变化情况。结果:与空白组相比,模型组大鼠血清D-乳酸含量升高(P0.01),空肠Gln浓度、Na+-K+-ATPase,Ca2+-ATPase活性降低(P0.01);平胃散治疗后各指标均有恢复,且效果都好于自然恢复组。结论:湿困脾胃证能造成肠黏膜屏障功能及离子泵出现障碍,平胃散对其有明显修复作用。

Functional Recovery from Damage of Intestinal Barrier and Ion Pump by Pingwei Powder in Rats with Syndrom of Dampness Blocking Spleen and Stomach

ObJective: To explore the damage of intestinal barrier in the rats model of dampness blocking spleen and stomach syndrome and the functional recovery of Pingwei powder for it. Method: SD rats were randomly divided into control group, model group, treatment group and autonomous recovery group, ten rats in each. Animal model of dampness blocking spleen and stomach was established. Such indexes as content of D-lactic acid and activities of DAO, Na⁺-K⁺-ATPase and Ca²⁺-ATPase were tested. Result: Compared with the blank group, the content of D-lactic acid increased (P<0.01)and the activities of Na⁺-K⁺-ATPase and Ca²⁺-ATPase decreased obviously(P<0.01)except DAO (P>0.05). The general trend in Pingwei powder group treatment was better than that in natural recovery. Conclusion: Pingwei Powder can markedly recovery the damage of intestinal barrier and ion pump in rats with syndrom of dampness blocking spleen and stomach.