小檗碱-四苯硼钠缔合纳米微粒体系的共振Rayleigh散射光谱及其分析应用小檗碱-四苯硼钠缔合纳米微粒体系的共振Rayleigh散射光谱及其分析应用

目的研究(小檗碱-四苯硼钠)_n缔合纳米微粒的形成与共振Rayleigh散射之间的关系，建立测定小檗碱的共振Rayleigh散射光谱分析新方法。方法采用共振Rayleigh散射光谱法、吸收光谱和透射电镜研究四苯硼钠(TPB)与盐酸小檗碱(BB)的缔合反应。结果在pH 5.0 NaAc-HAc缓冲溶液中，TPB与BB结合形成的(BB-TPB)_n缔合纳米微粒在470 nm处产生一个共振Rayleigh散射（RRS）峰。建立了测定0.06～5.28 mg·L^-1盐酸小檗碱的RRS新方法，检出限为26 μg·L^-1。 结论通过TPB－与BB⁺和Ag⁺反应形成可用透射电镜观测的(BB_jAg_p-TPBj+p)h复合缔合纳米微粒，证实了固液界面的形成是导致其共振Rayleigh散射光增强的充分必要条件。建立的RRS新方法可用于中成药中微量小檗碱的测定，具有灵敏度高，试样用量少等特点。

Resonance Rayleigh scattering spectra of berberine-tetraphenylboron association nanoparticle and its analytical application

AIM: To study the relationship between resonance Rayleigh scattering(RRS) and the formation of association nanoparticle, and to develop a new and sensitive RRS method for the determination of trace berberine (BB). METHODS: The association nanoparticle between BB and tetraphenylboron (TPB) was investigated by means of RRS, absorption spectral method and transmission electron microscope (TEM). RESULTS: In pH 5.0 NaAc-HAc buffer solution, BB and TPB combine to BB-TPB association complex. The association complexes aggregate to (BB-TPB)n association nanoparticles, due to the strong hydrophobic force and Van der Waals force. It exhibits a resonance Rayleigh scattering peak at 470 nm, a maximum absorption peak at 368 nm. A new RRS method was proposed for the determination of 0.06 to 5.28 mg.L-1 BB in real samples with satisfactory results. The detection limit is 26 micrograms.L-1. CONCLUSION: The TEM of (BBjAgp-TPBj + p)h composite association nanoparticles was observed by means of composite association nanoreaction both TPB- and BB+ or Ag+. The results indicated that the formation of (BB-TPB)n association nanoparticles and interface of solid-liquid results in the enhanced resonance light-scattering. This new RRS method showed high sensitivity, and had been successfully applied to the determination of BB in real samples.