葛根素对缺氧性血管内皮细胞凋亡的保护作用

目的 研究葛根素(puerarin)对缺氧性血管内皮细胞凋亡的影响。方法 用NaCN合并无糖培养基造成培养的牛主动脉内皮细胞(BAECs)缺氧;用台盼蓝染色、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)、流式细胞仪计数和Hoechst 33342荧光染色法观察细胞受损和凋亡情况;用免疫细胞化学染色法观察细胞内Caspase-3的表达情况。结果NaCN合并无糖培养基可引起血管内皮细胞凋亡。葛根素可显著减少缺氧性内皮细胞凋亡,并对Caspase-3的表达有明显抑制作用。结论葛根素对缺氧条件下的血管内皮细胞有保护作用,此作用至少部分通过抑制Caspase-3的表达而实现。

Protective effect of puerarin on vascular endothelial cell apoptosis induced by chemical hypoxia in vitro

AIM: To study the effect of puerarin on vascular endothelial cells apoptosis induced by chemical hypoxia-ischemia in vitro. METHODS: The chemical hypoxia-ischemia model was performed by treating cultured bovine aortic endothelial cells (BAECs) with NaCN in glucose-free medium. Cell viability was determined by trypan blue staining. Cell apoptosis was defined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), flow cytometry and Hoechst 33342 staining. The expression of Caspase-3 in endothelial cells was detected by immunocytochemical method. RESULTS: Chemical hypoxia-ischemia was shown to initiate bovine aortic endothelial cell apoptosis. Puerarin (0.5-3 mmol.L-1) was found to inhibit endothelial cell apoptosis effectively, and reduce the expression of Caspase-3 significantly. CONCLUSION: Puerarin can protect apoptotic endothelial cells induced by chemical hypoxia-ischemia markedly and the effect was performed partly by decreasing Caspase-3 expression.