九州虫草的核苷类成分及不同部位两种活性成分含量的分布九州虫草的核苷类成分及不同部位两种活性成分含量的分布

目的快速测定九州虫草中的部分核苷类成分及其主要活性成分虫草素与腺苷的含量，并考察九州虫草不同部位虫草素和腺苷含量的分布。方法用高效毛细管区带电泳法确定野生和人工九州虫草中的核苷类成分,测定虫草素和腺苷的含量，并比较它们在九州虫草不同部位含量的分布。结果蒙山九州虫草中含有至少8种核苷或碱基，其中抗肿瘤活性成分虫草素的含量远高于冬虫夏草和蛹虫草,且人工栽培九州虫草中虫草素的含量又显著高于野生九州虫草。腺苷主要存在于九州虫草子座中，而虫草素则不仅在九州虫草子座中含量甚高，人工栽培九州虫草虫体中虫草素含量也较高。结论天然和人工九州虫草及其不同部位的核苷和碱基成分存在一定差异，九州虫草中的虫草素含量高，具有良好的栽培、药用与开发前景。

Nucleoside from Cordyceps kyushuensis and the distribution of two active components in its different parts

AIM: To rapidly separate and determine the nucleosides from natural and cultured Cordyceps kyushuensis Kob., and to compare the content of cordycepin and adenosine in different parts of Cordyceps kyushuensis Kob., which are the main nucleoside active components in medicinal fungus belonging to Cordyceps (Fr.) Link. METHODS: The nucleosides were separated and determined by the high performance capillary zone electrophoresis (CZE). Beckman P/ACE system MDQ apparatus equipped with a PDA detector and a uncoated fused-silica capillary (41 cm x 45 microns ID, 30 cm effective length) were used. The experimental conditions were as follows: the running buffer was borax solution (adjust to pH 9.4 with sodium hydroxide), applied voltage was 20 kV, operated temperature was 20 degrees C and the detector wavelength was 258 nm. The content of cordycepin and adenosine in the fruiting body, stroma and host worm of natural and cultured C. kyushuensis were respectively investigated and quantitatively analyzed. RESULTS: There are at least 8 kinds of nucleoside or nitrogen base in Cordyceps kyushuensis Kob. The content of cordycepin which is a bio-active substance with anti-tumor activity in C. kyushuensis is significantly higher than that in C. sinensis and C. militaris, and furthermore the cordycepin in the cultured C. kyushuensis is notably higher than the natural one. Adenosine was mainly found from the stroma of C. kyushuensis, While the cordycepin content is high in the stroma of both natural and cultured C. kyushuensis as well as in the host worm of the cultured one. CONCLUSION: There are some differences about the nucleoside components between the natural and cultured C. kyushuensis and between the different parts of them. With a high cordycepin content, C. kyushuensis should have a considerable medicinal potential.